{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T10:38:56Z","timestamp":1785494336360,"version":"3.56.0"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T00:00:00Z","timestamp":1718755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["491454339"],"award-info":[{"award-number":["491454339"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2024,10]]},"abstract":"\n Objective<\/jats:title>\n To evaluate the potential utility of antibody\u2010drug conjugates targeting trophoblast cell surface antigen\u20102 (TROP\u20102) in patients with primary penile squamous cell carcinoma (PSCC), patients with recurrence (REC cohort), and patient\u2010matched distant metastases (MET cohort), and to assess the potential use of TROP\u20102 as a predictive non\u2010invasive biomarker in PSCC.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n A cohort comprising a PRIM (\n n<\/jats:italic>\n =\u200937), REC (\n n<\/jats:italic>\n =\u20095) and MET subcohort (\n n<\/jats:italic>\n =\u20097), with MET including lymph node and lung metastases, was analysed using quantitative real\u2010time PCR, ELISA and immunohistochemical staining with evaluation of H\u2010score.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n TROP\u20102 mRNA and serum protein levels were significantly increased in primary and recurrent PSCC compared to cancer\u2010free controls (both\n P<\/jats:italic>\n \u2009<\u20090.001). Immunohistochemical analysis revealed that most of the PRIM cohort (\n n<\/jats:italic>\n =\u200934\/37, median H\u2010score 260, interquartile range [IQR] 210\u2013300), as well as all patients in the REC (median [IQR] H\u2010score 200 [165\u2013290]) and MET cohorts (median [IQR] H\u2010score 280 [260\u2013300]) exhibited moderate to strong membranous TROP\u20102 expression. Additionally, The H\u2010score (membranous TROP\u20102 expression) was positively correlated with TROP\u20102 mRNA (\n \u03c1<\/jats:italic>\n =\u20090.69,\n P<\/jats:italic>\n <\u20090.0001,\n R<\/jats:italic>\n 2<\/jats:sup>\n =\u20090.70) and protein levels (\n \u03c1<\/jats:italic>\n =\u20090.86,\n P<\/jats:italic>\n <\u20090.0001,\n R<\/jats:italic>\n 2<\/jats:sup>\n =\u20090.59), indicating its potential as a non\u2010invasive biomarker in PSCC.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n In summary, our results support further studies on TROP\u20102 as a diagnostic and therapeutic target in primary, recurrent and metastatic PSCC.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/bju.16442","type":"journal-article","created":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T21:18:27Z","timestamp":1718831907000},"page":"615-621","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Trophoblast cell surface antigen\u20102: a promising new biomarker and potential therapeutic target in penile squamous cell carcinoma"],"prefix":"10.1111","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6562-5518","authenticated-orcid":false,"given":"Richard","family":"Weiten","sequence":"first","affiliation":[{"name":"Department of Urology Uro\u2010Oncology, Robot\u2010Assisted and Specialized Urologic Surgery University Hospital Cologne Cologne Germany"},{"name":"Department of Urology and Paediatric Urology University Hospital Bonn Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8250-5522","authenticated-orcid":false,"given":"Enno","family":"Storz","sequence":"additional","affiliation":[{"name":"Department of Urology Uro\u2010Oncology, Robot\u2010Assisted and Specialized Urologic Surgery University Hospital Cologne Cologne 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