{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T23:20:07Z","timestamp":1781133607641,"version":"3.54.1"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,11,16]],"date-time":"2024-11-16T00:00:00Z","timestamp":1731715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007568","name":"Urology Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007568","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020400","name":"John Black Charitable Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020400","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Radical prostatectomy (RP) represents the cornerstone of surgical treatment for prostate cancer. Assessing surgical margin status intraoperatively with current techniques remains challenging due to high costs in the context of an already stretched pathology workforce. Fluorescence confocal microscopy (FCM) is a promising technique to detect margins in prostate cancer surgery not bound by such limitations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design<\/jats:title>\n                    <jats:p>\n                      The Imperial Prostate 8 \u2013 Fluorescence Confocal Microscopy for Rapid Evaluation of Surgical Cancer Excision (IP8\u2010FLUORESCE) study is a multicentre, prospective,\n                      <jats:italic>ex vivo<\/jats:italic>\n                      , \u2018blinded\u2019, comparative cohort study. It aims to assess the accuracy of digital FCM for detection of prostate cancer at surgical margins compared to traditional histopathology.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Endpoints<\/jats:title>\n                    <jats:p>The primary endpoint is the accuracy of digital FCM for detection of prostate cancer at surgical margins on a per\u2010patient level, reported with sensitivity, specificity, positive and negative predictive values.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and Methods<\/jats:title>\n                    <jats:p>A total of 153 patients with localised prostate cancer undergoing robot\u2010assisted RP across three UK National Health Service tertiary referral centres will be recruited. Following RP, prostate specimens will undergo immediate immersion in Acridine Orange solution, scanning \u2018en face\u2019 with FCM using the Histolog\u00ae Scanner, and subsequent formalin fixation and paraffin embedding. Two independent, \u2018blinded\u2019 uro\u2010pathologists will report both the FCM images and the histopathology slides. Recruitment commenced on 17 August 2023.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bju.16588","type":"journal-article","created":{"date-parts":[[2024,11,16]],"date-time":"2024-11-16T07:40:49Z","timestamp":1731742849000},"page":"502-509","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Fluorescence confocal microscopy for margin assessment in prostatectomy:\n                    <scp>IP8<\/scp>\n                    \u2010\n                    <scp>FLUORESCE<\/scp>\n                    study protocol"],"prefix":"10.1111","volume":"135","author":[{"given":"Nikhil","family":"Mayor","sequence":"first","affiliation":[{"name":"Imperial Prostate, Division of Surgery, Department of Surgery and Cancer, Faculty of Medicine Imperial College London  London UK"},{"name":"Department of Urology Imperial College Healthcare National Health Service (NHS) Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7246-3651","authenticated-orcid":false,"given":"Alexander","family":"Light","sequence":"additional","affiliation":[{"name":"Imperial Prostate, Division of Surgery, Department of Surgery and Cancer, Faculty of Medicine Imperial College London  London UK"},{"name":"Department of Urology Imperial College Healthcare National Health Service (NHS) Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Silvanto","sequence":"additional","affiliation":[{"name":"Department of Pathology University College London Hospitals NHS Foundation Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Cullen","sequence":"additional","affiliation":[{"name":"Imperial Prostate, Division of Surgery, Department of Surgery and Cancer, Faculty of Medicine Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng Yun","family":"Ng","sequence":"additional","affiliation":[{"name":"Department of Urology Imperial College Healthcare National Health Service (NHS) Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Almostafa","family":"Badreldin","sequence":"additional","affiliation":[{"name":"Department of Urology Imperial College Healthcare National Health Service (NHS) Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bijan","family":"Khoubehi","sequence":"additional","affiliation":[{"name":"Department of Urology Imperial College Healthcare National Health Service (NHS) Trust  London UK"},{"name":"Department of Urology Chelsea and Westminster NHS Foundation Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giles","family":"Hellawell","sequence":"additional","affiliation":[{"name":"Department of Urology Imperial College Healthcare National Health Service (NHS) Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Fiorentino","sequence":"additional","affiliation":[{"name":"Nightingale\u2010Saunders Clinical Trials &amp; Epidemiology Unit, King's Clinical Trials Unit &amp; Division of Methodology King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin J.","family":"Connor","sequence":"additional","affiliation":[{"name":"Imperial Prostate, Division of Surgery, Department of Surgery and Cancer, Faculty of Medicine Imperial College London  London UK"},{"name":"Department of Urology Imperial College Healthcare National Health Service (NHS) Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1642-1208","authenticated-orcid":false,"given":"Taimur T.","family":"Shah","sequence":"additional","affiliation":[{"name":"Imperial Prostate, Division of Surgery, Department of Surgery and Cancer, Faculty of Medicine Imperial College London  London UK"},{"name":"Department of Urology Imperial College Healthcare National Health Service (NHS) Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1674-6723","authenticated-orcid":false,"given":"Hashim U.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Imperial Prostate, Division of Surgery, Department of Surgery and Cancer, Faculty of Medicine Imperial College London  London UK"},{"name":"Department of Urology Imperial College Healthcare National Health Service (NHS) Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Winkler","sequence":"additional","affiliation":[{"name":"Imperial Prostate, Division of Surgery, Department of Surgery and Cancer, Faculty of Medicine Imperial College London  London UK"},{"name":"Department of Urology Imperial College Healthcare National Health Service (NHS) Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,16]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"Reports \u2013 National Prostate Cancer Audit. Available at:https:\/\/www.npca.org.uk\/reports\/?audience%5B%5D=professional. Accessed May 2024"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2023.41.6_suppl.298"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euros.2024.05.003"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.15316"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euf.2022.10.002"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.15256"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.15771"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/S13063\u2010022\u201006421\u20107"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.15868"},{"key":"e_1_2_6_11_1","unstructured":"College report finds UK wide histopathology staff shortages. Available at:https:\/\/www.rcpath.org\/discover\u2010pathology\/news\/college\u2010report\u2010finds\u2010severe\u2010staff\u2010shortages\u2010across\u2010services\u2010vital\u2010to\u2010cancer\u2010diagnosis.html. Accessed January 2023"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpcy.68"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.15938"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2020.0476"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.19017"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.15108"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.16239"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61116-8"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0301653"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.16441"}],"container-title":["BJU International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bju.16588","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:32:15Z","timestamp":1761744735000},"score":1,"resource":{"primary":{"URL":"https:\/\/bjui-journals.onlinelibrary.wiley.com\/doi\/10.1111\/bju.16588"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,16]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1111\/bju.16588"],"URL":"https:\/\/doi.org\/10.1111\/bju.16588","archive":["Portico"],"relation":{},"ISSN":["1464-4096","1464-410X"],"issn-type":[{"value":"1464-4096","type":"print"},{"value":"1464-410X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,16]]},"assertion":[{"value":"2024-11-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}