{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T23:35:52Z","timestamp":1793144152599,"version":"4.5.0"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30CA240139"],"award-info":[{"award-number":["P30CA240139"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["U01CA239141"],"award-info":[{"award-number":["U01CA239141"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["U01CA27140"],"award-info":[{"award-number":["U01CA27140"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R01CA272766"],"award-info":[{"award-number":["R01CA272766"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To evaluate basal\u2010luminal cell of origin subtyping using Prediction Analysis of Microarray of 50 genes (PAM50) genomic classification profiles for predicting disease progression in men undergoing active surveillance (AS) for prostate cancer (PCa).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and Methods<\/jats:title>\n                    <jats:p>In the prospective Miami Active Surveillance Trial (MAST) trial, 205 men undergoing AS received serial multiparametric magnetic resonance imaging (MRI) and biopsies, including MRI\u2010targeted and systematic sampling. The highest\u2010grade core from each biopsy was sent for expression profiling using Decipher, a clinical\u2010grade transcriptome assay (Veracyte Inc., San Diego, CA, USA). Basal\u2010luminal subtyping was evaluated using PAM50 molecular subtype models. PCa grade progression was compared across subtypes, as were gene mutation signatures, prognostic indices, and pathway activities. Kaplan\u2013Meier curves, log\u2010rank test, and multivariable Cox regression were used to assess association between PAM50 and grade progression. Heatmaps and volcano plots were rendered to illustrate potential mechanistical differences between PAM50 subtypes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of the 205 patients, 128 had transcriptome data for baseline basal\u2010luminal classification. PAM50 identified 46 Luminal A (LA), 26 Luminal B (LB), and 56 Basal subtypes. Decipher scores were lowest in LA, followed by Basal, and highest in LB. Grade progression\u2010free survival was worse in patients with the LB subtype (median 1.7\u2009years) compared to those with LA and Basal subtypes (median 2.9\u2009years; log\u2010rank\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.005); LB patients had grade progression\u2010free survival of 34% by 24\u2009months of AS, compared to 63% for Basal or 68% for LA. Transcriptome analysis showed distinct enrichment profiles for each subtype, with LB strongly associated with\n                      <jats:italic>SPOP<\/jats:italic>\n                      and\n                      <jats:italic>CHD1<\/jats:italic>\n                      mutations. Limitations include small sample size and single\u2010institution setting.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The PAM50 basal\u2010luminal subtyping shows promise as a molecular classification tool for predicting progression risk in PCa. This is one of the only prospective studies evaluating PAM50 subtyping for predicting cancer progression in a cohort of men undergoing AS for PCa.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bju.70202","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:48:15Z","timestamp":1772995695000},"page":"886-894","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Prediction Analysis of Microarray of 50 genes (\n                    <scp>PAM50)<\/scp>\n                    classifier validated for predicting prostate cancer progression in active surveillance: Miami Active Surveillance Trial (\n                    <scp>MAST)<\/scp>"],"prefix":"10.1111","volume":"137","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1498-7443","authenticated-orcid":false,"given":"Ankur","family":"Malpani","sequence":"first","affiliation":[{"name":"Sylvester Comprehensive Cancer Center, Miller School of Medicine, Desai Sethi Urology Institute University of Miami  Miami FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4571-1944","authenticated-orcid":false,"given":"Jonathan T.","family":"Ryan","sequence":"additional","affiliation":[{"name":"Dr. Kiran C. Patel College of Allopathic Medicine (NSU MD)  Davie FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4491-8855","authenticated-orcid":false,"given":"Hui","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Public Health Sciences Sylvester Comprehensive Cancer Center  Miami FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0320-2914","authenticated-orcid":false,"given":"Nachiketh","family":"Soodana\u2010Prakash","sequence":"additional","affiliation":[{"name":"Sylvester Comprehensive Cancer Center, Miller School of Medicine, Desai Sethi Urology Institute University of Miami  Miami FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3177-3439","authenticated-orcid":false,"given":"Archan","family":"Khandekar","sequence":"additional","affiliation":[{"name":"Sylvester Comprehensive Cancer Center, Miller School of Medicine, Desai Sethi Urology Institute University of Miami  Miami FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3505-8232","authenticated-orcid":false,"given":"Tarek","family":"Ajami","sequence":"additional","affiliation":[{"name":"Sylvester Comprehensive Cancer Center, Miller School of Medicine, Desai Sethi Urology Institute University of Miami  Miami FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Williams","sequence":"additional","affiliation":[{"name":"Sylvester Comprehensive Cancer Center, Miller School of Medicine, Desai Sethi Urology Institute University of Miami  Miami FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe","family":"Szczotka","sequence":"additional","affiliation":[{"name":"Sylvester Comprehensive Cancer Center, Miller School of Medicine, Desai Sethi Urology Institute University of Miami  Miami FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Alshalalfa","sequence":"additional","affiliation":[{"name":"Research and Development Veracyte Inc.  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