{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T23:50:47Z","timestamp":1790985047696,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To evaluate the diagnostic performance of the Conformit\u00e9 Europ\u00e9enne [CE]\u2010certified microRNA\u2010371a\u20103p (miR371a) assay in patients undergoing inguinal exploration for small testicular masses of unknown histology in a real\u2010world setting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and Methods<\/jats:title>\n                    <jats:p>Between March 2023 and March 2024, 60 patients underwent inguinal exploration for testicular masses of unknown histology. For miR371a analysis, peripheral venous blood was obtained and sent to the Department of Molecular Pathology within 30\u2009min. The extracted miRNA was reverse\u2010transcribed into complementary DNA (cDNA) with miRNA\u2010specific stem\u2010loop primers, followed by a pre\u2010amplification step. Using quantitative polymerase chain reaction, the miR371a is quantified. The median crossing points of the triplicates was calculated and converted into a relative quantification value.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The evaluation of the miR371a assay showed a true\u2010positive result in 25 patients with seminoma. Eight patients with seminoma had false\u2010negative results. In the non\u2010seminoma group, six of seven (86%) patients had a true positive result. The false\u2010negative finding derived from a non\u2010seminomatous germ cell tumour (NSGCT) with predominant teratoma. The test was true\u2010negative in 16 patients with sarcoma, diffuse large B\u2010cell lymphoma, Leydig cell tumour, and Sertoli cell tumour. False\u2010positive results were obtained in four patients. The results yield a sensitivity of 77.5%, a specificity of 80% and a positive predictive value of 88.6% and negative predictive value of 64% for the miR371a test in our cohort. In the seminoma cohort, the sensitivity of the serum tumour markers was 12%. In the NSGCT cohort, the sensitivity was 66%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>In the absence of suitable markers, the miR371a assay shows promising results in the preoperative diagnosis of suspected localised small testicular tumour. Standardisation of pre\u2010analytical handling and prospective multicentre validation are essential to define its optimal role in preoperative decision\u2010making.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bju.70215","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T12:35:02Z","timestamp":1773405302000},"page":"914-919","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Diagnostic performance of the microRNA\u2010371a assay in small testicular masses: a real\u2010world cohort study"],"prefix":"10.1111","volume":"137","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8411-8353","authenticated-orcid":false,"given":"Felix","family":"Seelemeyer","sequence":"first","affiliation":[{"name":"Uniklinik Cologne, Department of Urology  Cologne 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