{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T02:44:26Z","timestamp":1782787466248,"version":"3.54.5"},"reference-count":13,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T00:00:00Z","timestamp":1774656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T00:00:00Z","timestamp":1774656000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To determine the diagnostic performance of\n                      <jats:italic>ex vivo<\/jats:italic>\n                      fluorescence confocal microscopy (FCM) for prostate cancer detection in magnetic resonance imaging (MRI)\u2010targeted biopsy specimens using final histopathology as the reference standard, and to quantify workflow component times relevant to same\u2010day implementation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and Methods<\/jats:title>\n                    <jats:p>\n                      In this single\u2010centre prospective observational study, men undergoing MRI\u2010targeted transperineal prostate biopsy were enrolled. Targeted biopsy cores were imaged\n                      <jats:italic>ex vivo<\/jats:italic>\n                      using a Histolog\u00ae FCM scanner (SamanTree Medical SA, Lausanne, Switzerland) after rapid acridine orange immersion and prior to routine histopathological processing. Two board\u2010certified genitourinary pathologists independently reviewed FCM images in a blinded fashion and classified each specimen as positive or negative for prostate cancer and clinically significant cancer (CSC), defined as International Society of Urological Pathology Grade Group (GG) \u22652. Diagnostic performance was calculated against final histopathology. Workflow feasibility was assessed by measuring specimen preparation, scanning, and interpretation times. Interobserver agreement and exploratory discordance analyses were performed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 50 MRI\u2010targeted biopsy specimens from 44 patients were analysed, of which 33 (66%) contained prostate cancer and 17 (52%) met criteria for CSC. For cancer detection, Reader 1 achieved 87.9% sensitivity and 94.1% specificity, and Reader 2 achieved 97.0% sensitivity and 76.5% specificity, with overall accuracy of 90% for both readers. Interobserver agreement for cancer detection was 88% (\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u2009=\u20090.74), and for CSC was 86% (\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u2009=\u20090.61). Mean specimen preparation and interpretation times were ~2\u2009min each, with standardised scanning time of 50\u2009s, enabling complete FCM assessment in &lt;5\u2009min per target. Discordance was concentrated in GG 2 disease and Prostate Imaging\u2010Reporting and Data System (PI\u2010RADS) 3 targets, while higher\u2010grade cancers and PI\u2010RADS 5 lesions were uniformly concordant.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Ex vivo<\/jats:italic>\n                      FCM provides rapid, accurate, and reproducible assessment of MRI\u2010targeted prostate biopsy specimens for cancer detection within clinically feasible timeframes. These findings support FCM as a same\u2010day diagnostic adjunct and justify prospective trials evaluating real\u2010time implementation during targeted biopsy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bju.70263","type":"journal-article","created":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T09:54:05Z","timestamp":1774691645000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Same\u2010day fluorescence confocal microscopy of\n                    <scp>MRI<\/scp>\n                    \u2010targeted prostate biopsies: A feasibility study"],"prefix":"10.1111","volume":"138","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6596-9100","authenticated-orcid":false,"given":"Salim K.","family":"Younis","sequence":"first","affiliation":[{"name":"Glickman Urological Institute Cleveland Clinic  Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7744-6190","authenticated-orcid":false,"given":"Mohamad","family":"Watfa","sequence":"additional","affiliation":[{"name":"Glickman Urological Institute Cleveland Clinic  Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"Glickman Urological Institute Cleveland Clinic  Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5426-9867","authenticated-orcid":false,"given":"Karim","family":"Daher","sequence":"additional","affiliation":[{"name":"Glickman Urological Institute Cleveland Clinic  Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas A.","family":"Soputro","sequence":"additional","affiliation":[{"name":"Glickman Urological Institute Cleveland Clinic  Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2579-0534","authenticated-orcid":false,"given":"Abdulrahman","family":"Al\u2010Bayati","sequence":"additional","affiliation":[{"name":"Glickman Urological Institute Cleveland Clinic  Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samarpit","family":"Rai","sequence":"additional","affiliation":[{"name":"Glickman Urological Institute Cleveland Clinic  Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui M.","family":"Bernardino","sequence":"additional","affiliation":[{"name":"Glickman Urological Institute Cleveland Clinic  Cleveland OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruben","family":"Olivares","sequence":"additional","affiliation":[{"name":"Glickman Urological Institute Cleveland Clinic  Cleveland OH 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