{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T02:44:26Z","timestamp":1782787466820,"version":"3.54.5"},"reference-count":21,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bjui-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJU International"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To demonstrate the reliability and diagnostic accuracy of fluorescence confocal microscopy (FCM) analysis of surgical margins during minimally invasive radical prostatectomy (RP).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and Methods<\/jats:title>\n                    <jats:p>From January 2022 to February 2023, we enrolled 100 consecutive patients in this single\u2010centre, prospective, non\u2010randomised trial (C2021\/32). The VivaScope\u00ae 2500M\u2010G4 microscope (Mavig GmbH, Munich, Germany), a laser scanning microscope designed for rapid acquisition of high\u2010resolution digital images, was employed. Following surgery, each posterolateral prostatic margin was analysed using FCM and reviewed by two expert urological pathologists. This study aimed to assess the accuracy of FCM in evaluating surgical margins during RP, comparing the diagnostic accuracy of FCM to final anatomical pathology (AP) and assessing the inter\u2010rater agreement between two urological pathologists (Cohen's kappa agreement).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 100 patients (i.e., 200 surgical margins) were included. Overall, 37 margins in 33 patients were positive according to FCM. At final pathology, 38 specimens in 29 patients were positive. On a per\u2010patient analysis sensitivity, specificity, positive predictive value, and negative predictive value of FCM were 65.5%, 80.2%, 57.5%, and 85.0%, respectively. On a per\u2010margin analysis, they were 60.5%, 91.3%, 62.1%, and 90.7%, respectively. The inter\u2010rater agreement was 0.64 and 0.61 for per\u2010patient and per\u2010margin analysis, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Fluorescence confocal microscopy is a feasible technology to be employed during RP, offering a good diagnostic accuracy without distorting final AP.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bju.70264","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T16:11:39Z","timestamp":1774973499000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Margin evaluation with fluorescence confocal microscopy during minimally invasive radical prostatectomy"],"prefix":"10.1111","volume":"138","author":[{"given":"Josep M.","family":"Gaya","sequence":"first","affiliation":[{"name":"Department of Urology Fundaci\u00f3 Puigvert  Barcelona Spain"},{"name":"Institut de Recerca Sant Pau (IR SANT PAU)  Barcelona Spain"},{"name":"Department of Surgery Autonomous University of Barcelona  Bellaterra Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3662-6828","authenticated-orcid":false,"given":"Paolo","family":"Verri","sequence":"additional","affiliation":[{"name":"Department of Urology Fundaci\u00f3 Puigvert  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3316-5691","authenticated-orcid":false,"given":"Andrea","family":"Gallioli","sequence":"additional","affiliation":[{"name":"Department of Urology Fundaci\u00f3 Puigvert  Barcelona Spain"},{"name":"Institut de Recerca Sant Pau (IR SANT PAU)  Barcelona 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