{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T09:42:58Z","timestamp":1762249378179},"reference-count":75,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,4,4]],"date-time":"2012-04-04T00:00:00Z","timestamp":1333497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The molecular mechanisms underlying the regulation of gap junction (GJ) channels based on the 43\u2010kDa connexin isoform (Cx43) have been studied extensively. GJ channels are formed by the docking of opposed hemichannels in adjacent cells. Mounting data indicate that unopposed Cx43 hemichannels are also functional in the plasma membrane. However, our understanding of how Cx43\u2010hemichannel opening and closing is regulated at the molecular level is only poorly understood. Recent work elucidated that actomyosin contractility inhibits potently Cx43 hemichannels. It is known that intracellular Ca<jats:sup>2+<\/jats:sup> exerts a bell\u2010shaped\u2010dependent effect on Cx43\u2010hemichannel opening. While low\u2010intracellular [Ca<jats:sup>2+<\/jats:sup>] (&lt;500 nM) provokes opening of the channel, high\u2010intracellular [Ca<jats:sup>2+<\/jats:sup>] (&gt; 500 nM) favours closing of the channel. The mechanism underlying this negative regulation of Cx43\u2010hemichannel activity by high\u2010intracellular [Ca<jats:sup>2+<\/jats:sup>] seems to be dependent on the activation of the actomyosin contractile system. The activity of Cx43 hemichannels is critically controlled by molecular interactions between the intracellular loop and the C\u2010terminal tail. These interactions are essential for Cx43\u2010hemichannel opening in response to triggers such as cytosolic [Ca<jats:sup>2+<\/jats:sup>] rise or external [Ca<jats:sup>2+<\/jats:sup>] lowering. In this review, we present the hypothesis that the actomyosin contractile system can function as an important brake mechanism on Cx43\u2010hemichannel opening. By controlling loop\u2013tail interactions, the contractile system would prevent aberrant or excessive opening of Cx43 hemichannels.<\/jats:p>","DOI":"10.1111\/boc.201100079","type":"journal-article","created":{"date-parts":[[2012,2,29]],"date-time":"2012-02-29T10:55:41Z","timestamp":1330512941000},"page":"367-377","source":"Crossref","is-referenced-by-count":32,"title":["The contractile system as a negative regulator of the connexin 43 hemichannel"],"prefix":"10.1111","volume":"104","author":[{"given":"Raf","family":"Ponsaerts","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Himpens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc","family":"Leybaert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geert","family":"Bultynck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,4,4]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"\u2606Articles of special interest"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb908"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00295.2003"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-009-0007-5"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.039594"},{"key":"e_1_2_8_7_1","first-page":"383","article-title":"In vivo cellular delivery of siRNA","volume":"13","author":"Brink P.R.","year":"2010","journal-title":"Drugs"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.82.9.929"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201101055"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.200800236"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2010.07.018"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0770"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600908"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2009.07.002"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2011.05.007"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2008.196"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2003.12.030"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-1582"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M207016200"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.34.21867"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20060175"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/09687680210139839"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002801"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.45.29745"},{"key":"e_1_2_8_25_1","article-title":"Functional consequences of abnormal Cx43 expression in the heart","author":"Fontes M.S.","year":"2011","journal-title":"Biochim. 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