{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:22:15Z","timestamp":1790968935914,"version":"4.1.0"},"reference-count":65,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,11,9]],"date-time":"2012-11-09T00:00:00Z","timestamp":1352419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background information<\/jats:title><jats:p>Several host proteins play crucial roles in the HIV\u20101 replication cycle. The endosomal sorting complex required for transport (ESCRT) exemplifies a large, multi\u2010component host machinery that is required by HIV\u20101 for viral budding. ESCRT promotes the inward budding of vesicles from the membranes of late endosomes to generate multi\u2010vesicular bodies. However, HIV\u20101 co\u2010opts the ESCRT to enable outwards budding of virus particles from the plasma membrane, a phenomenon that is topologically similar to multi\u2010vesicular body biogenesis. A role for ESCRTII in mRNA trafficking has been established in <jats:italic>Drosophila<\/jats:italic> in which the ESCRT\u2010II components, Vps22 and Vps36, promote the localisation of the bicoid mRNA in the fertilised egg. This is achieved via specific interactions with the Staufen protein. In this work, we investigated a possible implication of ESCRT\u2010II in the HIV\u20101 replication cycle.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Co\u2010immunoprecipitation analyses and live cell tri\u2010molecular fluorescence complementation assays revealed that interactions between EAP30 and Gag and another between EAP30 and Staufen1 occur in mammalian cells. We then depleted EAP30 (the orthologue for Vps22) by siRNA to target ESCRT\u2010II in HIV\u20101 expressing cells. This treatment disrupted ESCRT\u2010II function and leads to the degradation of the two other ESCRT\u2010II complex proteins, EAP45 and EAP20, as well as the associated Rab7\u2010interacting lysosomal protein. The depletion of EAP30 led to dramatically reduced viral structural protein Gag and virus production levels, without any effect on viral RNA levels. On the contrary, the overexpression of EAP30 led to a several\u2010fold increase in virus production. Unexpec\u2010tedly, siRNA\u2010mediated depletion of EAP30 led to a block to HIV\u20101 genomic RNA trafficking and resulted in the accumulation of genomic RNA in the nucleus and juxtanuclear domains.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our data provide the first evidence that the Staufen1\u2013ESCRT\u2010II interaction is evolutionarily conserved from lower to higher eukaryotes and reveal a novel role for EAP30 in the control of HIV\u20101 RNA trafficking and gene expression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/boc.201200021","type":"journal-article","created":{"date-parts":[[2012,9,15]],"date-time":"2012-09-15T03:40:33Z","timestamp":1347680433000},"page":"706-721","source":"Crossref","is-referenced-by-count":30,"title":["ESCRT\u2010II's involvement in HIV\u20101 genomic RNA trafficking and 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