{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T09:15:28Z","timestamp":1786871728227,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,4,24]],"date-time":"2016-04-24T00:00:00Z","timestamp":1461456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005802","name":"Autoritatea Nationala pentru Cercetare Stiintifica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005802","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec><jats:title>Background Information<\/jats:title><jats:p>Atherosclerosis is an inflammatory disease, in which risk factors such as hyperlipidemia and hypertension affect the arterial endothelium, resulting in dysfunction, cell damage or both. The number of circulating endothelial progenitor cells and microparticles provides invaluable outcome prediction for atherosclerosis disease. However, evidence for the therapeutic potential of endothelial progenitor cells and microparticles in atherosclerosis development is limited. Our study was designed to investigate the possible protective role of a cell therapy\u2010based approach, using endothelial progenitor cells and the dual behaviour of circulating platelet microparticles, on atherosclerosis development in hypertensive\u2010hypercholesterolemic hamster model. Consequently, control hamsters received four intravenous inoculations of: <jats:bold>(1)<\/jats:bold> 1\u00d710<jats:sup>5<\/jats:sup> endothelial progenitor cells of healthy origins in one dose per month, during four months of diet\u2010induced atherosclerosis, and after hypertensive\u2010hypercholesterolemic diet for further four months; <jats:bold>(2)<\/jats:bold> in a second set of experiments, 1\u00d710<jats:sup>5<\/jats:sup> endothelial progenitor cells of healthy origins or\/and 1\u00d710<jats:sup>5<\/jats:sup> platelet microparticles of atherosclerotic origins were inoculated every other month during hypertensive\u2010hypercholesterolemic diet.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Endothelial progenitor cell treatment had the following effects: <jats:bold>(1)<\/jats:bold> re\u2010established plasmatic parameters: cholesterol and triglyceride concentrations, blood pressure, heart rate, cytokine and chemokine profiles, platelet microparticle pro\u2010thrombotic activity and endothelial progenitor cell paracrine activity reflected by cytokine\/chemokine detection; <jats:bold>(2)<\/jats:bold> reduced lipid, macrophage and microparticle accumulation in liver; <jats:bold>(3)<\/jats:bold> reduced atherosclerosis development, revealed by decreased lipid, macrophage and microparticle content of arterial wall; <jats:bold>(4)<\/jats:bold> induced the recruitment and incorporation of endothelial progenitor cells into liver and arterial wall; <jats:bold>(5)<\/jats:bold> improved arterial dysfunction by increasing contraction and relaxation; <jats:bold>(6)<\/jats:bold> reduced the protein expression of specific pro\u2010inflammatory molecules in liver and arterial wall. Platelet microparticle transplantation aggravated the above\u2010mentioned biomarkers and atherosclerosis process, which were partially reverted with co\u2010inoculation of platelet microparticles and endothelial progenitor cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>With this study, we demonstrate in a hypertensive\u2010hypercholesterolemic hamster model, that the endothelial progenitor cell\u2010based therapy suppresses the development of atherosclerosis and reduces hepatic lipid and macrophage accumulation with the consequent alleviation of dyslipidaemia and hypertension.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance<\/jats:title><jats:p>Our results support the notion that increasing the number of circulating endothelial progenitor cells by different ways could be a promising therapeutic tool for atherosclerosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/boc.201500104","type":"journal-article","created":{"date-parts":[[2016,3,12]],"date-time":"2016-03-12T07:27:59Z","timestamp":1457767679000},"page":"219-243","source":"Crossref","is-referenced-by-count":34,"title":["Effects of transplanted circulating endothelial progenitor cells and platelet microparticles in atherosclerosis development"],"prefix":"10.1111","volume":"108","author":[{"given":"Adriana","family":"Georgescu","sequence":"first","affiliation":[{"name":"Institute of Cellular Biology and Pathology \u2018Nicolae Simionescu\u2019 of Romanian Academy  Bucharest Romania"},{"name":"Instituto de Medicina Molecular Faculdade de Medicina da Universidade de Lisboa  Lisboa Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicoleta","family":"Alexandru","sequence":"additional","affiliation":[{"name":"Institute of Cellular Biology and Pathology \u2018Nicolae Simionescu\u2019 of Romanian Academy  Bucharest Romania"},{"name":"Instituto de Medicina Molecular Faculdade de Medicina da Universidade de Lisboa  Lisboa Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugen","family":"Andrei","sequence":"additional","affiliation":[{"name":"Institute of Cellular Biology and Pathology \u2018Nicolae Simionescu\u2019 of Romanian Academy  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuel","family":"Dragan","sequence":"additional","affiliation":[{"name":"Institute of Cellular Biology and Pathology \u2018Nicolae Simionescu\u2019 of Romanian Academy  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Cochior","sequence":"additional","affiliation":[{"name":"Faculty of Medicine \u2018Titu Maiorescu\u2019 University  Bucharest Romania"},{"name":"General Surgery Clinic CF 2 Clinical Hospital  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio","family":"Dias","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular Faculdade de Medicina da Universidade de Lisboa  Lisboa Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2010.04122.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052058"},{"key":"e_1_2_10_4_1","first-page":"5866","article-title":"Endothelial precursor cells as a model of tumor endothelium: characterization and comparison with mature endothelial cells","volume":"63","author":"Bagley R.G.","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2005.04.007"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003752"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-008-0710-9"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-12-4103"},{"key":"e_1_2_10_9_1","first-page":"153","article-title":"Dismutation of eosinophil superoxide by mast cell granule superoxide dismutase","volume":"5","author":"Dileepan K.N.","year":"1989","journal-title":"Biochem. 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