{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T15:41:47Z","timestamp":1769269307774,"version":"3.49.0"},"reference-count":86,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,7,31]],"date-time":"2022-07-31T00:00:00Z","timestamp":1659225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Ezrin protein is involved in the interaction of actin cytoskeleton with membrane receptors such as CD44. It regulates plasma membrane dynamics and intracellular signaling. <jats:italic>Coxiella burnetii<\/jats:italic>, the etiologic agent of Q fever, is internalized into host cell through a poorly characterized molecular mechanism. Here we analyzed the role of ezrin and CD44 in the <jats:italic>C. burnetii<\/jats:italic> internalization by HeLa cells. The knockdown of ezrin and CD44 inhibited the bacterial uptake. Interestingly, at early stages of <jats:italic>C. burnetii<\/jats:italic> internalization, ezrin was recruited to the cell membrane fraction and phosphorylated. Moreover, the overexpression of non\u2010phosphorylatable and phosphomimetic ezrin mutants decreased and increased the bacterial entry, respectively. A decrease in the internalization of <jats:italic>C. burnetii<\/jats:italic> was observed by the overexpression of CD44 truncated forms containing the intracellular or the extracellular domains. Interestingly, the CD44 mutant was unable to interact with ERM proteins decreased the bacterial internalization. These findings demonstrate the participation of ezrin in the internalization process of <jats:italic>C. burnetii<\/jats:italic> in non\u2010phagocytic cells. Additionally, we present evidence that CD44 receptor would be involved in that process.<\/jats:p><\/jats:sec>","DOI":"10.1111\/boc.202100096","type":"journal-article","created":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T10:18:40Z","timestamp":1658225920000},"page":"237-253","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Ezrin and CD44 participate in the internalization process of <i>Coxiella burnetii<\/i> into non\u2010phagocytic cells"],"prefix":"10.1111","volume":"114","author":[{"given":"Jes\u00fas S.","family":"Distel","sequence":"first","affiliation":[{"name":"Instituto de Histolog\u00eda y Embriolog\u00eda Facultad de Ciencias M\u00e9dicas Universidad Nacional de Cuyo \u2010 CONICET  Mendoza Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodolfo M.","family":"Ortiz Flores","sequence":"additional","affiliation":[{"name":"Instituto de Histolog\u00eda y Embriolog\u00eda Facultad de Ciencias M\u00e9dicas Universidad Nacional de Cuyo \u2010 CONICET  Mendoza Argentina"},{"name":"C\u00e1tedra de Microbiolog\u00eda Parasitolog\u00eda e Inmunolog\u00eda Facultad de Odontolog\u00eda Universidad Nacional de Cuyo  Mendoza Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur","family":"Bienvenu","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM) UMR 9004 CNRS Universit\u00e9 de Montpellier  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milton O.","family":"Aguilera","sequence":"additional","affiliation":[{"name":"Instituto de Histolog\u00eda y Embriolog\u00eda Facultad de Ciencias M\u00e9dicas Universidad Nacional de Cuyo \u2010 CONICET  Mendoza Argentina"},{"name":"C\u00e1tedra de Microbiolog\u00eda Parasitolog\u00eda e Inmunolog\u00eda Facultad de Odontolog\u00eda Universidad Nacional de Cuyo  Mendoza Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matteo","family":"Bonazzi","sequence":"additional","affiliation":[{"name":"Institut de Recherche en Infectiologie de Montpellier (IRIM) UMR 9004 CNRS Universit\u00e9 de Montpellier  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9654-5467","authenticated-orcid":false,"given":"Walter","family":"Ber\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto de Histolog\u00eda y Embriolog\u00eda Facultad de Ciencias M\u00e9dicas Universidad Nacional de Cuyo \u2010 CONICET  Mendoza Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,7,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.07.016"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90694-A"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.47.2.127-149.1983"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00175\u201011"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2011.02.039"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/21.5.893"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"6078","DOI":"10.4049\/jimmunol.163.11.6078","article-title":"Subversion of monocyte functions by Coxiella burnetii: impairment of the cross\u2010talk between alphavbeta3 integrin and CR3","volume":"163","author":"Capo C.","year":"1999","journal-title":"Journal of Immunology"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002056"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-4683"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.589523"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.317354.118"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e06-12-1144"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.1.160-164.2000"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2015.00175"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00045-16"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115.6.1231"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00411-16"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200307032"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.4172\/2157\u20107439.1000316"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21041502"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.6.8.1061"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.1.193"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003107"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.5.3315-3322.2001"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02178-14"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200807047"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7872"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.9.5963"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2012.07.006"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.380972"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108654200"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01912"},{"key":"e_1_2_9_34_1","article-title":"ERM proteins: the missing actin linkers in clathrin\u2010mediated endocytosis","volume":"307272","author":"Kvalvaag A.S.","year":"2018","journal-title":"bioRxiv"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986611794927910"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200316936"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)70277-5"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2006.10.075"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.204842"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.6994"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1159\/000489072"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.43.25324"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2011.01158.x"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004013"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.140.3.647"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.11.5527-5533.1998"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.494872"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199609)168:3<638::AID-JCP16>3.0.CO;2-V"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06744-7"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.13066"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2018.01.009"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc0700133"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep04034"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80836-3"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.22759"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830250228"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/1757-2215-7-40"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1004"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.17.4565"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.215541"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20061383"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/9042851"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039348"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145211"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2016.05.009"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.112.13.2059"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2000.00028.x"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01208-15"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-017-0430-9"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1007777"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01096-07"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1256070"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1356540"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00954"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00695-19"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00738"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.37262"},{"key":"e_1_2_9_78_1","volume-title":"Molecular and cellular biology of phagocytosis","author":"Tuckwell D.S.","year":"2020"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-09-3808"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-02-076539"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3049"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2013.1607"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-7-95"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1039\/b820576k"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.145.7.1497"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21020435"}],"container-title":["Biology of the Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/boc.202100096","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/boc.202100096","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/boc.202100096","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T07:26:48Z","timestamp":1692775608000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/boc.202100096"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,31]]},"references-count":86,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["10.1111\/boc.202100096"],"URL":"https:\/\/doi.org\/10.1111\/boc.202100096","archive":["Portico"],"relation":{},"ISSN":["0248-4900","1768-322X"],"issn-type":[{"value":"0248-4900","type":"print"},{"value":"1768-322X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,31]]},"assertion":[{"value":"2021-12-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}