{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:26:58Z","timestamp":1753878418931,"version":"3.41.2"},"reference-count":55,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,11]],"date-time":"2022-12-11T00:00:00Z","timestamp":1670716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background Information<\/jats:title><jats:p>Various types of stress initially induce a state of cardiac hypertrophy (CH) in the heart. But, persistent escalation of cardiac stress leads to progression from an adaptive physiological to a maladaptive pathological state. So, elucidating molecular mechanisms that can attenuate CH is imperative in developing cardiac therapies. Previously, we showed that Prohibitin1 (PHB1) has a protective role in CH\u2010induced oxidative stress. Nevertheless, it is unclear how PHB1, a mitochondrial protein, has a protective role in CH. Therefore, we hypothesized that PHB1 maintains mitochondrial quality in CH. To test this hypothesis, we used Isoproterenol (ISO) to induce CH in H9C2 cells overexpressing PHB1 and elucidated mitochondrial quality control pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that overexpressing PHB1 attenuates ISO\u2010induced CH and restores mitochondrial morphology in H9C2 cells. In addition, PHB1 blocks the pro\u2010hypertrophic IGF1R\/AKT pathway and restores the mitochondrial membrane polarization in ISO\u2010treated cells. We observed that overexpressing PHB1 promotes mitochondrial biogenesis, improves mitochondrial respiratory capacity, and triggers mitophagy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that PHB1 maintains mitochondrial quality in ISO\u2010induced CH in H9C2 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance<\/jats:title><jats:p>Based on our results, we suggest that small molecules that induce PHB1 in cardiac cells may prove beneficial in developing cardiac therapies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/boc.202200094","type":"journal-article","created":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T15:12:52Z","timestamp":1669907572000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prohibitin1 maintains mitochondrial quality in isoproterenol\u2010induced cardiac hypertrophy in H9C2 cells"],"prefix":"10.1111","volume":"115","author":[{"given":"Moumita","family":"Chakrabarti","sequence":"first","affiliation":[{"name":"Applied Biology Department CSIR\u2010Indian Institute of Chemical Technology  Hyderabad Telangana India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ganesh Kumar","family":"Raut","sequence":"additional","affiliation":[{"name":"Applied Biology Department CSIR\u2010Indian Institute of Chemical Technology  Hyderabad Telangana India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3260-2543","authenticated-orcid":false,"given":"Nishant","family":"Jain","sequence":"additional","affiliation":[{"name":"Applied Biology Department CSIR\u2010Indian Institute of Chemical Technology  Hyderabad Telangana 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