{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:27:02Z","timestamp":1753878422916,"version":"3.41.2"},"reference-count":56,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T00:00:00Z","timestamp":1724025600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82060247"],"award-info":[{"award-number":["82060247"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004479","name":"Natural Science Foundation of Jiangxi Province","doi-asserted-by":"publisher","award":["20202BAB206027","20232ACB206020"],"award-info":[{"award-number":["20202BAB206027","20232ACB206020"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Background information: The purinergic ligand\u2010gated ion channel 7 receptor (P2X7R) is an ATP\u2010gated ion channel that transmits extracellular signals and induces corresponding biological effects, transient receptor potential vanilloid type 1 (TRPV1) is a non\u2010selective cation channel that maintains normal physiological functions; numerous studies showed that P2X7R and TRPV1 are associated with inflammatory reactions. Results: The effect of P2X7R knockdown in satellite glial cells (SGCs) on neuronal TRPV1 expression under high glucose and high free fat (HGHF) environment was investigated. P2X7 short hairpin RNA (shRNA) was utilized to downregulate P2X7R in SGCs, and treated and untreated SGCs were co\u2010cultured with neuronal cell lines. The expression levels of inflammatory factors and signaling pathways in SGCs and neurons were measured using Western blot analysis, RT\u2010qPCR, immunofluorescence, and enzyme\u2010linked immunosorbent assays. Results suggested that P2X7 shRNA reduced the expression levels of P2X7R protein and mRNA in SGCs surrounding DRG neurons and downregulated the release of tumor necrosis factor\u2010alpha and interleukin\u20101 beta via the Ca<jats:sup>2+<\/jats:sup>\/p38 MAPK\/NF\u2010\u03baB pathway. Additionally, the downregulation of P2X7R might decrease TRPV1 expression in neurons via the Ca<jats:sup>2+<\/jats:sup>\/PKC\u2010\u025b\/p38 MAPK pathway.Conclusions: Reducing P2X7R expression in SCGs in an HGHF environment could decrease neuronal TRPV1 expression via the Ca<jats:sup>2+<\/jats:sup>\/PKC\u2010\u025b\/p38 MAPK pathway.<\/jats:p>","DOI":"10.1111\/boc.202400021","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T21:04:58Z","timestamp":1724101498000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reduction of TRPV1 expression on neurons due to downregulation of P2X7R in neonatal rat dorsal root ganglion satellite glial cells under co\u2010culture conditions"],"prefix":"10.1111","volume":"116","author":[{"given":"Hongji","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Physiology Basic Medical College of Nanchang University  Nanchang P.R. China"},{"name":"College of Economics and Management Shanghai Ocean University  Shanghai P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisha","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Physiology Basic Medical College of Nanchang University  Nanchang P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juping","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Physiology Basic Medical College of Nanchang University  Nanchang P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangchao","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Physiology Basic Medical College of Nanchang University  Nanchang P.R. 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