{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T11:11:08Z","timestamp":1790334668688,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Satellite cells are the primary drivers of skeletal muscle growth and regeneration and are a major cell source in both muscle tissue engineering and cultivated meat. However, current bovine satellite cell isolation can be technically demanding or yield heterogeneous cell populations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study presents a workflow optimized for simplicity and cost\u2010efficiency by integrating rapid muscle processing using electric mincing with magnetic\u2010activated cell sorting targeting Integrin\u2010\u03b17 to reliably enrich satellite cells. The isolated cells were characterized by Pax7 immunostaining and myogenic differentiation assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>ITGA7\u2010based magnetic sorting yielded an enriched satellite cell population corresponding to 49.39 \u00b1 14.70 \u00d7 10\u00b3 cells per gram of minced tissue. The isolated cells\u00a0exhibited high Pax7 expression (98 \u00b1 1.34% Pax7\u2010positive cells) and strong myogenic differentiation capacity (differentiation index: 94.39 \u00b1 3.58%), confirming their identity and functional potential.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>By minimizing hands\u2010on effort and avoiding specialized equipment, this workflow lowers technical and financial barriers while enabling the acquisition of highly enriched, early\u2010passage satellite cell populations. This workflow provides an adaptable foundation for advancing cultivated meat bioprocess development and accelerating research in skeletal muscle biology.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/boc.70079","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T12:15:41Z","timestamp":1788351341000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Streamlined Workflow for the Isolation of Bovine Satellite Cells From Muscle Tissue"],"prefix":"10.1111","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2216-8050","authenticated-orcid":false,"given":"Tobias Horst","family":"Baldeweg","sequence":"first","affiliation":[{"name":"Reutlingen Research Institute Reutlingen University  Reutlingen Germany"},{"name":"Institute of Interfacial Process Engineering and Plasma Technology (IGVP)  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svenja","family":"Nellinger","sequence":"additional","affiliation":[{"name":"Institute of Interfacial Process Engineering and Plasma Technology (IGVP)  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2229-4882","authenticated-orcid":false,"given":"Anna","family":"Leikeim","sequence":"additional","affiliation":[{"name":"Institute of Interfacial Process Engineering and Plasma Technology (IGVP)  Stuttgart Germany"},{"name":"Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Heine","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra Juliane","family":"Kluger","sequence":"additional","affiliation":[{"name":"Institute of Interfacial Process Engineering and Plasma Technology (IGVP)  Stuttgart Germany"},{"name":"Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB  Stuttgart Germany"},{"name":"School of Life Sciences Reutlingen University  Reutlingen 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