{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T04:46:57Z","timestamp":1790484417596,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,4,14]],"date-time":"2014-04-14T00:00:00Z","timestamp":1397433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["AG025688"],"award-info":[{"award-number":["AG025688"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["NIAP01AG1449"],"award-info":[{"award-number":["NIAP01AG1449"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["AG05136"],"award-info":[{"award-number":["AG05136"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["P30NS055077"],"award-info":[{"award-number":["P30NS055077"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000957","name":"Alzheimer's Association","doi-asserted-by":"publisher","award":["NIRG-12-242297"],"award-info":[{"award-number":["NIRG-12-242297"]}],"id":[{"id":"10.13039\/100000957","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["AG038259"],"award-info":[{"award-number":["AG038259"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Brain Pathology"],"published-print":{"date-parts":[[2014,7]]},"abstract":"Abstract<\/jats:title>\n \n We recently discovered that protein components of the ribonucleic acid (\n RNA<\/jats:styled-content>\n ) spliceosome form cytoplasmic aggregates in\n A<\/jats:styled-content>\n lzheimer's disease (\n AD<\/jats:styled-content>\n ) brain, resulting in widespread changes in\n RNA<\/jats:styled-content>\n splicing. However, the involvement of small nuclear\n RNAs<\/jats:styled-content>\n (\n snRNAs<\/jats:styled-content>\n ), also key components of the spliceosome complex, in the pathology of\n AD<\/jats:styled-content>\n remains unknown. Using immunohistochemical staining of post\u2010mortem human brain and spinal cord, we identified cytoplasmic tangle\u2010shaped aggregates of\n snRNA<\/jats:styled-content>\n in both sporadic and familial\n AD<\/jats:styled-content>\n cases but not in aged controls or other neurodegenerative disorders. Immunofluorescence using antibodies reactive with the 2,2,7\u2010trimethylguanosine cap of\n snRNAs<\/jats:styled-content>\n and transmission electron microscopy demonstrated\n snRNA<\/jats:styled-content>\n localization with tau and paired helical filaments, the main component of neurofibrillary tangles. Quantitative real\u2010time polymerase chain reaction (\n PCR<\/jats:styled-content>\n ) showed\n U<\/jats:styled-content>\n 1\n snRNA<\/jats:styled-content>\n accumulation in the insoluble fraction of\n AD<\/jats:styled-content>\n brains whereas other\n U snRNAs<\/jats:styled-content>\n were not enriched. In combination with our previous results, these findings demonstrate that aggregates of\n U<\/jats:styled-content>\n 1\n snRNA<\/jats:styled-content>\n and\n U<\/jats:styled-content>\n 1 small nuclear ribonucleoproteins represent a new pathological hallmark of\n AD<\/jats:styled-content>\n .\n <\/jats:p>","DOI":"10.1111\/bpa.12133","type":"journal-article","created":{"date-parts":[[2014,2,26]],"date-time":"2014-02-26T07:33:14Z","timestamp":1393399994000},"page":"344-351","source":"Crossref","is-referenced-by-count":91,"title":["Aggregates of Small Nuclear Ribonucleic Acids (\n snRNAs<\/scp>\n ) in\n A<\/scp>\n lzheimer's Disease"],"prefix":"10.1111","volume":"24","author":[{"given":"Chadwick M.","family":"Hales","sequence":"first","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University Atlanta GA"},{"name":"Department of Neurology Emory University School of Medicine Atlanta GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric B.","family":"Dammer","sequence":"additional","affiliation":[{"name":"Department of Neurology Emory University School of Medicine Atlanta GA"},{"name":"Department of Human Genetics Emory University School of Medicine Atlanta GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Diner","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University Atlanta GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Yi","sequence":"additional","affiliation":[{"name":"Robert P. Apkarian Integrated Electron Microscopy Core Emory University Atlanta GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas T.","family":"Seyfried","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University Atlanta GA"},{"name":"Department of Biochemistry Emory University School of Medicine Atlanta GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marla","family":"Gearing","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University Atlanta GA"},{"name":"Department of Pathology Emory University School of Medicine Atlanta GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan D.","family":"Glass","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University Atlanta GA"},{"name":"Department of Neurology Emory University School of Medicine Atlanta GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Montine","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Washington Seattle WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan I.","family":"Levey","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University Atlanta GA"},{"name":"Department of Neurology Emory University School of Medicine Atlanta GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James J.","family":"Lah","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University Atlanta GA"},{"name":"Department of Neurology Emory University School of Medicine Atlanta GA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,4,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1310249110"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awq258"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi201215r"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-30-09697.2003"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410410211"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2011.00024"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr900474t"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1326-6-82"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.111"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01386-5"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.20.9415"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/WNR.0b013e3280148e8b"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2011.10.030"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2012.12.042"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.9.1565"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2009.08.010"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1066"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(98)01337-X"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600701"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201202303"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000354170"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.02.009"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5065-07.2008"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215540104900301"}],"container-title":["Brain 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