{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:05:04Z","timestamp":1790553904323,"version":"4.1.0"},"reference-count":10,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T00:00:00Z","timestamp":1616371200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T00:00:00Z","timestamp":1616371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJCR18H3"],"award-info":[{"award-number":["JPMJCR18H3"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007428","name":"Naito Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP18ek0109391"],"award-info":[{"award-number":["JP18ek0109391"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain Pathology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Prion\u2010like spreading of abnormal proteins is proposed to occur in neurodegenerative diseases, and the progression of \u03b1\u2010synuclein (\u03b1\u2010syn) deposits has been reported in the brains of animal models injected with synthetic \u03b1\u2010syn fibrils or pathological \u03b1\u2010syn prepared from patients with Parkinson's disease (PD) and dementia with Lewy bodies (DLB). However, \u03b1\u2010syn transmission in nonhuman primates, which are more similar to humans, has not been fully clarified. Here, we injected synthetic human \u03b1\u2010syn fibrils into the left striatum of a macaque monkey (<jats:italic>Macaca fuscata<\/jats:italic>). At 3\u00a0months after the injection, we examined neurodegeneration and \u03b1\u2010syn pathology in the brain using \u03b1\u2010syn epitope\u2010specific antibodies, antiphosphorylated \u03b1\u2010syn antibodies (pSyn#64 and pSer129), anti\u2010ubiquitin antibodies, and anti\u2010p62 antibodies. Immunohistochemical examination with pSyn#64, pSer129, and \u03b1\u2010syn epitope\u2010specific antibodies revealed Lewy bodies, massive \u03b1\u2010syn\u2010positive neuronal intracytoplasmic inclusions (NCIs), and neurites in the left putamen. These inclusions were also positive for ubiquitin and p62. LB509, a human\u2010specific \u03b1\u2010syn antibody targeting amino acid residues 115\u2013122, showed limited immunoreactivity around the injection site. The left substantia nigra (SN) and the bilateral frontal cortex also contained some NCIs and neurites. The left hemisphere, including parietal\/temporal cortex presented sparse \u03b1\u2010syn pathology, and no immunoreactivity was seen in olfactory nerves, amygdala, hippocampus, or right parietal\/temporal cortex. Neuronal loss and gliosis in regions with \u03b1\u2010syn pathology were mild, except for the left striatum and SN. Our results indicate that abnormal \u03b1\u2010syn fibrils propagate throughout the brain of <jats:italic>M. fuscata<\/jats:italic> via projection, association, and commissural fibers, though the progression of \u03b1\u2010syn pathology is limited.<\/jats:p>","DOI":"10.1111\/bpa.12952","type":"journal-article","created":{"date-parts":[[2021,3,23]],"date-time":"2021-03-23T05:49:51Z","timestamp":1616478591000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Progression of phosphorylated \u03b1\u2010synuclein in <i>Macaca fuscata<\/i>"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1989-2398","authenticated-orcid":false,"given":"Ito","family":"Kawakami","sequence":"first","affiliation":[{"name":"Dementia Research Project Tokyo Metropolitan Institute of Medical Science Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuko","family":"Motoda","sequence":"additional","affiliation":[{"name":"Department of Clinical Neuroscience &amp; Therapeutics Hiroshima University Graduate School of Biomedical and Health Sciences Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Dementia Research Project Tokyo Metropolitan Institute of Medical Science Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aki","family":"Shimozawa","sequence":"additional","affiliation":[{"name":"Dementia Research Project Tokyo Metropolitan Institute of Medical Science Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masami","family":"Masuda\u2010Suzukake","sequence":"additional","affiliation":[{"name":"Dementia Research Project Tokyo Metropolitan Institute of Medical Science Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reiko","family":"Ohtani","sequence":"additional","affiliation":[{"name":"Dementia Research Project Tokyo Metropolitan Institute of Medical Science Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mina","family":"Takase","sequence":"additional","affiliation":[{"name":"Dementia Research Project Tokyo Metropolitan Institute of Medical Science Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Kumashiro","sequence":"additional","affiliation":[{"name":"Brain Science Institute Tamagawa University Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuyuki","family":"Samejima","sequence":"additional","affiliation":[{"name":"Brain Science Institute Tamagawa University Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Dementia Research Project Tokyo Metropolitan Institute of Medical Science Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/35081564"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-009-0538-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-4580(02)00065-9"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awt037"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-017-0413-0"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1902279116"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.01.037"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-014-0088-8"},{"key":"e_1_2_8_10_1","volume-title":"The rhesus monkey brain in stereotaxic coordinates","author":"George Paxinos X\u2010FH","year":"2008"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1787-16.2017"}],"container-title":["Brain Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bpa.12952","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/bpa.12952","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bpa.12952","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bpa.12952","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T08:36:46Z","timestamp":1692347806000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bpa.12952"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,22]]},"references-count":10,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.1111\/bpa.12952"],"URL":"https:\/\/doi.org\/10.1111\/bpa.12952","archive":["Portico"],"relation":{},"ISSN":["1015-6305","1750-3639"],"issn-type":[{"value":"1015-6305","type":"print"},{"value":"1750-3639","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,22]]},"assertion":[{"value":"2020-12-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12952"}}