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Inflammasome activation has been suggested to play a critical role in the neuroinflammatory response in AD progression, but the cell\u2010type expression of inflammasome proteins in the brain has not been fully characterized. In this study, we used samples from the hippocampus formation, the subiculum, and the entorhinal cortex brain from 17 donors with low\u2010level AD pathology and 17 intermediate AD donors to assess the expression of inflammasome proteins. We performed analysis of hippocampal thickness, \u03b2\u2010amyloid plaques, and hyperphosphorylated tau to ascertain the cellular pathological changes that occur between low and intermediate AD pathology. Next, we determined changes in the cells that express the inflammasome sensor proteins NOD\u2010like receptor proteins (NLRP) 1 and 3, and caspase\u20101. In addition, we stained section with IC100, a humanized monoclonal antibody directed against the inflammasome adaptor protein apoptosis\u2010associated speck\u2010like protein containing a caspase recruitment domain (ASC), and a commercially available anti\u2010ASC antibody. Our results indicate that hippocampal cortical thickness did not significantly change between low and intermediate AD pathology, but there was an increase in pTau and \u03b2\u2010amyloid clusters in intermediate AD cases. NLRP3 was identified mainly in microglial populations, whereas NLRP1 was seen in neuronal cytoplasmic regions. There was a significant increase of ASC in neurons labeled by IC100, whereas microglia in the hippocampus and subiculum were labeled with the commercial anti\u2010ASC antibody. Caspase\u20101 was present in the parenchyma in the CA regions where amyloid and pTau were identified. Together, our results indicate increased inflammasome protein expression in the early pathological stages of AD, that IC100 identifies neurons in early stages of AD and that ASC expression correlates with A\u03b2 and pTau in postmortem AD brains.<\/jats:p>","DOI":"10.1111\/bpa.13142","type":"journal-article","created":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T09:13:48Z","timestamp":1672305228000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Identification of inflammasome signaling proteins in neurons and microglia in early and intermediate stages of Alzheimer's disease"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2664-8265","authenticated-orcid":false,"given":"Regina T.","family":"Vontell","sequence":"first","affiliation":[{"name":"Department of Neurology University of Miami Brain Endowment Bank, University of Miami Miller School of Medicine  Miami Florida USA"},{"name":"Evelyn F. 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