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Fox Foundation for Parkinson's Research","doi-asserted-by":"publisher","award":["MJFF\u2010019033"],"award-info":[{"award-number":["MJFF\u2010019033"]}],"id":[{"id":"10.13039\/100000864","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain Pathology"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Parkinson's disease (PD), multiple system atrophy (MSA), and dementia with Lewy bodies (DLB) are neurodegenerative disorders with alpha\u2010synuclein (\u03b1\u2010syn) aggregation pathology. Different strains of \u03b1\u2010syn with unique properties are suggested to cause distinct clinical and pathological manifestations resulting in PD, MSA, or DLB. To study individual \u03b1\u2010syn spreading patterns, we injected \u03b1\u2010syn fibrils amplified from brain homogenates of two MSA patients and two PD patients into the brains of C57BI6\/J mice. Antibody staining against pS129\u2010\u03b1\u2010syn showed that \u03b1\u2010syn fibrils amplified from the brain homogenates of the four different patients caused different levels of \u03b1\u2010syn spreading. The strongest \u03b1\u2010syn pathology was triggered by \u03b1\u2010syn fibrils of one of the two MSA patients, followed by comparable pS129\u2010\u03b1\u2010syn induction by the second MSA and one PD patient material. Histological analysis using an antibody against Iba1 further showed that the formation of pS129\u2010\u03b1\u2010syn is associated with increased microglia activation. In contrast, no differences in dopaminergic neuron numbers or co\u2010localization of \u03b1\u2010syn in oligodendrocytes were observed between the different groups. Our data support the spreading of \u03b1\u2010syn pathology in MSA, while at the same time pointing to spreading heterogeneity between different patients potentially driven by individual patient immanent factors.<\/jats:p>","DOI":"10.1111\/bpa.13196","type":"journal-article","created":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T04:15:21Z","timestamp":1690172121000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Alpha\u2010synuclein fibrils amplified from multiple system atrophy and Parkinson's disease patient brain spread after intracerebral injection into mouse brain"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7645-5448","authenticated-orcid":false,"given":"Shuyu","family":"Zhang","sequence":"first","affiliation":[{"name":"Clinical Department of Neurology, School of Medicine, University Hospital rechts der Isar Technical University of Munich  Munich 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