{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:29:00Z","timestamp":1753878540719,"version":"3.41.2"},"reference-count":10,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T00:00:00Z","timestamp":1706140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014667","name":"British Neuropathological Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014667","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain Pathology"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Formalin\u2010fixed paraffin\u2010embedded (FFPE) brain tissue held in tissue banks constitutes a valuable research resource, especially when associated with clinical annotations and longitudinal psychometric testing. Apolipoprotein\u2010E (APOE) genotyping is important to fully characterise this resource, however older FFPE tissue may not be suitable for genotyping. We performed polymerase chain reaction restriction fragment length polymorphism (PCR\u2010RFLP) assays on DNA extracted from post\u2010mortem FFPE brain tissue ranging from 2\u201019\u2009years old. A maximum of three years in paraffin was determined for robust APOE genotyping of FFPE tissue using PCR\u2010RFLP which may suggest prolonged storage of fixed tissue as FFPE blocks may have deleterious effects on DNA.<\/jats:p>","DOI":"10.1111\/bpa.13243","type":"journal-article","created":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T12:01:41Z","timestamp":1706184101000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Apolipoprotein\u2010E genotyping in formalin\u2010fixed and paraffin\u2010embedded post\u2010mortem brain tissue"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9372-5163","authenticated-orcid":false,"given":"James","family":"Minshull","sequence":"first","affiliation":[{"name":"Faculty of Biology Medicine and Health, School of Biological Sciences, Division of Neuroscience The University of Manchester  Manchester UK"},{"name":"Geoffrey Jefferson Brain 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