{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T08:04:23Z","timestamp":1780473863117,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain Pathology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Diffuse hemispheric glioma, H3 G34\u2010mutant (DHG\u2010H3 G34) has been primarily molecularly characterized by methylation profiling and sequencing studies. We describe an integrated histomolecular evaluation including high\u2010resolution copy number profiling of a series of 60 DHG\u2010H3 G34 to further our understanding of the spectrum of genetic changes associated with this tumor type. Cases were clinically tested using an 187\u2010gene mutation and fusion targeted neuro\u2010oncology next\u2010generation sequencing panel (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200960) and Oncoscan chromosomal microarray (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200926) by a single laboratory (2018\u20132022). A subset of cases had immunohistochemical results for OLIG2 (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200942), p53 (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200948), and ATRX (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200946), and methylation array data (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20098). Median age at testing was 21\u2009years (range, 12\u201350). No significant difference was noted in clinical, histopathological, and mutational profile between pediatric and adult patients.\n                    <jats:italic>H3\u20103A<\/jats:italic>\n                    G34 mutations included G34R (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200956; 94%), G34V (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20093), and a non\u2010canonical G34E (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20091). Concurrent mutations most often involved\n                    <jats:italic>TP53<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200955; 92%),\n                    <jats:italic>ATRX<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200950; 83%), and\n                    <jats:italic>PDGFRA<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200934; 57%). A reportedly primary tumor was confirmed to be hypermutant and had a\n                    <jats:italic>PMS2<\/jats:italic>\n                    mutation. A single case also showed an\n                    <jats:italic>FGFR3::FAM184B<\/jats:italic>\n                    fusion. All cases with available chromosomal microarray data had unbalanced genomes, which were often complex (14\/26; 54%). The most frequent recurrent copy number abnormalities were losses involving 3q, 4q, 10q, 13q, and 18q, and 17p copy\u2010neutral loss of heterozygosity (cnLOH) encompassing\n                    <jats:italic>TP53<\/jats:italic>\n                    . This copy number profile was reminiscent of that seen in Grade 4 IDH\u2010mutant astrocytomas. Collectively, a\n                    <jats:italic>TP53<\/jats:italic>\n                    abnormality at copy number (12\/26, all cnLOH), sequence (55\/60) and protein expression (46\/48) level was detected in all 60 cases. In conclusion, integrated high\u2010resolution copy number and histomolecular analysis expanded the spectrum of genetic changes associated with DHG\u2010H3 G34, including the presence of universal\n                    <jats:italic>TP53<\/jats:italic>\n                    abnormalities with frequent cnLOH\u2014a copy number abnormality that has been largely unrecognized\u2014for this new 2021 World Health Organization central nervous system tumor type.\n                  <\/jats:p>","DOI":"10.1111\/bpa.70076","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T11:57:56Z","timestamp":1770379076000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated high\u2010resolution copy number and histomolecular analysis of diffuse hemispheric glioma,\n                    <scp>H3 G34<\/scp>\n                    \u2010mutant reveals universal\n                    <i>TP53<\/i>\n                    abnormalities"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5346-7753","authenticated-orcid":false,"given":"Jorge A.","family":"Trejo\u2010Lopez","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0434-4965","authenticated-orcid":false,"given":"Cinthya Zepeda","family":"Mendoza","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M.","family":"Kollmeyer","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristen L.","family":"Drucker","sequence":"additional","affiliation":[{"name":"Department of Neurologic Surgery Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa M.","family":"Pazdernik","sequence":"additional","affiliation":[{"name":"Department of Quantitative Health Sciences Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A.","family":"Decker","sequence":"additional","affiliation":[{"name":"Department of Quantitative Health Sciences Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne E.","family":"Praska","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayson","family":"Hardcastle","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Smoley","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Isaacson","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mallika","family":"Gandham","sequence":"additional","affiliation":[{"name":"Department of Quantitative Health Sciences Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surendra","family":"Dasari","sequence":"additional","affiliation":[{"name":"Department of Quantitative Health Sciences Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aditya","family":"Raghunathan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Giannini","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Wagner","sequence":"additional","affiliation":[{"name":"Department of Pathology Orlando Regional Medical Center  Orlando Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8661-6727","authenticated-orcid":false,"given":"M. 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