{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:03:20Z","timestamp":1790017400577,"version":"4.0.1"},"reference-count":63,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain Pathology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Neuritic plaques (NP) are a key component of Alzheimer disease (AD) pathology composed of dystrophic neurites that form neurofibrillary tangles (NFTs) and amyloid\u2010\n                    <jats:italic>\u03b2<\/jats:italic>\n                    (A\n                    <jats:italic>\u03b2<\/jats:italic>\n                    ) proteins. NP accumulation is one fundamental feature seen in the intermediate stage of AD neuropathological pathology change. NPs are sites for cellular degeneration and can induce the upregulation of inflammatory signals including the inflammasome complex. We analyzed 758 genes using multiplex genomics in samples from the hippocampal, temporal and frontal brain regions that had intermediate AD neuropathological changes and aged\u2010matched controls. In addition, we analyzed NP formation using\n                    <jats:italic>phosphorylated tau at threonine 217<\/jats:italic>\n                    (pTau217) and A\n                    <jats:italic>\u03b2<\/jats:italic>\n                    antibodies along with inflammasome sensors using NOD\u2010like receptor protein (NLRP) antibodies,\n                    <jats:italic>apoptosis\u2010associated speck like protein containing a caspase recruitment domain<\/jats:italic>\n                    (ASC) and\n                    <jats:italic>absent in melanoma\u2010like receptor 2<\/jats:italic>\n                    (AIM2). Finally, we investigated if cell death is occurring cells by pyroptosis using Gasdermin D (GSDMD) antibody to detect pore formation on the membrane. Analyses show that inflammasome signaling genes and proteins are significantly increased in the hippocampus rather than the temporal and frontal lobe at the intermediate stage of AD. NPs are more prevalent in the hippocampus and temporal lobe in cases with intermediate AD pathology. ASC (1C100) immunostaining is colocalized with NLRP1, NLRP3 and AIM2. GSDMD immunostaining detected pericellular pores forming around the membrane of NFTs and in NPs. Messenger RNA and protein analyses demonstrate that inflammasome signaling molecules are elevated in AD suggesting that neuronal death occurs by the induction of pyroptosis.\n                  <\/jats:p>","DOI":"10.1111\/bpa.70086","type":"journal-article","created":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T23:50:56Z","timestamp":1771804256000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Increased expression of inflammasome signaling genes and proteins in selective brain regions in the intermediate stage of Alzheimer's disease"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5460-8834","authenticated-orcid":false,"given":"Juan Pablo","family":"de Rivero Vaccari","sequence":"first","affiliation":[{"name":"Department of Neurological Surgery and The Miami Project to Cure Paralysis University of Miami Miller School of Medicine  Miami Florida USA"},{"name":"Department of Cellular Physiology and Molecular Biophysics University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Miami Brain Endowment Bank, University of Miami Miller School of Medicine  Miami Florida USA"},{"name":"Department of Neurology University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew P.","family":"Sawaya","sequence":"additional","affiliation":[{"name":"Dr. Phillip Frost Department of Dermatology &amp; Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna P.","family":"Garamszegi","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Miami Brain Endowment Bank, University of Miami Miller School of Medicine  Miami Florida USA"},{"name":"Department of Neurology University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Miami Brain Endowment Bank, University of Miami Miller School of Medicine  Miami Florida USA"},{"name":"Department of Neurology University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayled","family":"Barreda","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Miami Brain Endowment Bank, University of Miami Miller School of Medicine  Miami Florida USA"},{"name":"Department of Neurology University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen M.","family":"Bramlett","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery and The Miami Project to Cure Paralysis University of Miami Miller School of Medicine  Miami Florida USA"},{"name":"Bruce W. Carter Department of Veterans Affairs Medical Center  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakir Humayun","family":"Gultekin","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Miami Brain Endowment Bank, University of Miami Miller School of Medicine  Miami Florida USA"},{"name":"Department of Neurology University of Miami Miller School of Medicine  Miami Florida USA"},{"name":"Department of Pathology University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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