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Using a neonatal rhesus macaque SIV model, we tracked early brain\u2010associated viral DNA\/RNA, neuropathology, and cerebrospinal fluid (CSF) metabolism, and evaluated the impact of early antiretroviral therapy (ART). CSF viral RNA was detectable by 2\u2009days post infection (dpi), mirroring plasma viral load kinetics. In contrast, brain tissue SIV DNA and RNA remained largely undetectable through the first 3\u2009days of infection. Despite this early viral dissemination, we observed no significant neuropathological lesions or CSF proinflammatory cytokine responses during the first week. However, marked CSF metabolic dysregulation emerged by 1 month post infection. Initiating ART early, specifically at 3 dpi, normalized these neurometabolic abnormalities. These findings demonstrate that CSF metabolomic shifts can occur independently of overt neuroinflammation during the early stages of viral seeding. 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