{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T20:48:28Z","timestamp":1762375708095},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,9,17]],"date-time":"2013-09-17T00:00:00Z","timestamp":1379376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2013,10]]},"abstract":"Background and Purpose<\/jats:title>Chronic ethanol abuse and haemorrhagic shock are major causes of global mortality and, separately, induce profound hepato\u2010 and immune\u2010toxic effects via activation of NF<\/jats:styled-content>\u2010\u03baB<\/jats:styled-content>. Here, we assessed the effects of chronic ethanol intake upon the pathophysiological derangements after haemorrhagic shock with subsequent resuscitation (H<\/jats:styled-content>\/R<\/jats:styled-content>), with particular attention to the contribution of NF<\/jats:styled-content>\u2010\u03baB<\/jats:styled-content>.<\/jats:p><\/jats:sec>Experimental Approach<\/jats:title>Transgenic NF\u2010\u03baBEGFP<\/jats:sup> mice, expressing the enhanced green fluorescent protein (EGFP)<\/jats:styled-content> under the transcriptional control of NF<\/jats:styled-content>\u2010\u03baB<\/jats:styled-content> cis\u2010elements were fed a L<\/jats:styled-content>ieber\u2010D<\/jats:styled-content>eC<\/jats:styled-content>arli diet containing ethanol (EtOH\u2010diet) or an isocaloric control diet for 4 weeks and were then pairwise subjected to H<\/jats:styled-content>\/R<\/jats:styled-content>. Liver tissues and peripheral blood were sampled at 2 or 24\u2009h after H\/R. Cytokines in blood and tissue and leukocyte activation (as CD11b expression) were measured, along with EGFP as a marker of NF\u2010\u03baB activation.<\/jats:p><\/jats:sec>Key Results<\/jats:title>The EtOH\u2010diet increased mortality at 24\u2009h after H<\/jats:styled-content>\/R<\/jats:styled-content> and elevated liver injury, associated with an up\u2010regulation of NF<\/jats:styled-content>\u2010\u03baB<\/jats:styled-content>\u2010dependent genes and IL<\/jats:styled-content>\u20106 release; it also increased production of NF<\/jats:styled-content>\u2010\u03baB<\/jats:styled-content>\u2010driven intercellular adhesion molecule 1 (ICAM<\/jats:styled-content>\u20101) and EGFP<\/jats:styled-content> in liver tissue. At 2h after the H<\/jats:styled-content>\/R<\/jats:styled-content> procedure in ethanol\u2010fed mice we observed the highest proportion of NF<\/jats:styled-content>\u2010\u03baB<\/jats:styled-content> activated non\u2010parenchymal cells and an NF<\/jats:styled-content>\u2010\u03baB<\/jats:styled-content>\u2010dependent increase in polymorphonuclear leukocyte CD<\/jats:styled-content>11b expression.<\/jats:p><\/jats:sec>Conclusions and Implications<\/jats:title>The EtOH\u2010diet exacerbated liver injury after H<\/jats:styled-content>\/R<\/jats:styled-content>, accompanying an overwhelming hepatic and systemic immune response. Our findings contribute to evidence implicating NF<\/jats:styled-content>\u2010\u03baB<\/jats:styled-content> as a key player in the orchestration of the immune response in haemorrhagic shock patients with a history of chronic ethanol abuse.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.12224","type":"journal-article","created":{"date-parts":[[2013,5,3]],"date-time":"2013-05-03T14:28:17Z","timestamp":1367591297000},"page":"506-518","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Activation of NF\u2010\u03baB<\/scp> after chronic ethanol intake and haemorrhagic shock\/resuscitation in mice"],"prefix":"10.1111","volume":"170","author":[{"given":"M","family":"Maraslioglu","sequence":"first","affiliation":[{"name":"Department of Trauma Surgery Johann Wolfgang Goethe\u2010University Frankfurt (Main) 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