{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T03:35:21Z","timestamp":1774409721644,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,3,4]],"date-time":"2014-03-04T00:00:00Z","timestamp":1393891200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2014,3]]},"abstract":"Background and Purpose<\/jats:title>Spinocerebellar ataxias (SCAs<\/jats:styled-content>) are a family of chronic progressive neurodegenerative diseases, clinically and genetically heterogeneous, characterized by loss of balance and motor coordination due to degeneration of the cerebellum and its afferent and efferent connections. Unlike other motor disorders, the possible role of changes in the endocannabinoid system in the pathogenesis of SCAs<\/jats:styled-content> has not been investigated.<\/jats:p><\/jats:sec>Experimental Approach<\/jats:title>The status of cannabinoid receptor type 1 (CB1<\/jats:sub><\/jats:styled-content>) and cannabinoid receptor type 2 (CB2<\/jats:sub><\/jats:styled-content>) receptors in the post\u2010mortem cerebellum of SCA<\/jats:styled-content> patients and controls was investigated using immunohistochemical procedures.<\/jats:p><\/jats:sec>Key Results<\/jats:title>Immunoreactivity for the CB1<\/jats:sub><\/jats:styled-content> receptor, and also for the CB2<\/jats:sub><\/jats:styled-content> receptor, was found in the granular layer, P<\/jats:styled-content>urkinje cells, neurons of the dentate nucleus and areas of white matter in the cerebellum of SCA patients at levels notably higher than controls. Double\u2010labelling procedures demonstrated co\u2010localization of CB1<\/jats:sub><\/jats:styled-content> and, in particular, CB2<\/jats:sub><\/jats:styled-content> receptors with calbindin, supporting the presence of these receptors in P<\/jats:styled-content>urkinje neurons. Both receptors also co\u2010localized with Iba\u20101 and glial fibrillary acidic protein in the granular layer and white matter areas, indicating that they are present in microglia and astrocytes respectively.<\/jats:p><\/jats:sec>Conclusions and Implications<\/jats:title>Our results demonstrate that CB1<\/jats:sub><\/jats:styled-content> and CB2<\/jats:sub><\/jats:styled-content> receptor levels are significantly altered in the cerebellum of SCA<\/jats:styled-content> patients. Their identification in P<\/jats:styled-content>urkinje neurons, which are the main cells affected in SCAs<\/jats:styled-content>, as well as the changes they experienced, suggest that alterations in endocannabinoid receptors may be related to the pathogenesis of SCAs<\/jats:styled-content>. Therefore, the endocannabinoid system could provide potential therapeutic targets for the treatment of SCAs<\/jats:styled-content> and its progression.<\/jats:p><\/jats:sec>Linked Articles<\/jats:title>This article is part of a themed section on Cannabinoids 2013. To view the other articles in this section visit http:\/\/dx.doi.org\/10.1111\/bph.2014.171.issue\u20106<\/jats:ext-link><\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.12283","type":"journal-article","created":{"date-parts":[[2013,8,14]],"date-time":"2013-08-14T14:31:08Z","timestamp":1376490668000},"page":"1472-1489","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Changes in CB1<\/sub><\/scp> and CB2<\/sub><\/scp> receptors in the post\u2010mortem cerebellum of humans affected by spinocerebellar ataxias"],"prefix":"10.1111","volume":"171","author":[{"given":"Carmen","family":"Rodr\u00edguez\u2010Cueto","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular Instituto Universitario de Investigaci\u00f3n en Neuroqu\u00edmica Facultad de Medicina Universidad Complutense Madrid Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Enfermedades 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