{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:45:57Z","timestamp":1787013957299,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,9,17]],"date-time":"2013-09-17T00:00:00Z","timestamp":1379376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2013,10]]},"abstract":"\n Background and Purpose<\/jats:title>\n \n PDE<\/jats:styled-content>\n 4 inhibition suppresses experimental autoimmune encephalomyelitis (\n EAE<\/jats:styled-content>\n ), an animal model of multiple sclerosis (\n MS<\/jats:styled-content>\n ). However, side effects hinder\n PDE<\/jats:styled-content>\n 4 inhibitors clinical use.\n PDE<\/jats:styled-content>\n 7 inhibition might constitute an alternative therapeutic strategy, but few data about the anti\u2010inflammatory potential of\n PDE<\/jats:styled-content>\n 7 inhibitors are currently available. We have used the\n EAE<\/jats:styled-content>\n model to perform a comparative evaluation of\n PDE<\/jats:styled-content>\n 4 and\n PDE<\/jats:styled-content>\n 7 inhibition as strategies for\n MS<\/jats:styled-content>\n treatment.\n <\/jats:p>\n <\/jats:sec>\n \n Experimental Approach<\/jats:title>\n \n Two\n PDE<\/jats:styled-content>\n 7 inhibitors, the sulfonamide derivative\n BRL<\/jats:styled-content>\n 50481 and the recently described quinazoline compound\n TC<\/jats:styled-content>\n 3.6, were assayed to modulate\n EAE<\/jats:styled-content>\n in\n SJL<\/jats:styled-content>\n mice, in comparison with the well\u2010known\n PDE<\/jats:styled-content>\n 4 inhibitor Rolipram. We evaluated clinical signs, presence of inflammatory infiltrates in\n CNS<\/jats:styled-content>\n and anti\u2010inflammatory markers. We also analysed the effect of these inhibitors on the inflammatory profile of spleen cells\n in vitro<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Key Results<\/jats:title>\n \n TC<\/jats:styled-content>\n 3.6 prevented\n EAE<\/jats:styled-content>\n with efficacy similar to Rolipram, while\n BRL<\/jats:styled-content>\n 50481 had no effect on the disease. Differences between both\n PDE<\/jats:styled-content>\n 7 inhibitors are discussed. Data from Rolipram and\n TC<\/jats:styled-content>\n 3.6 showed that\n PDE<\/jats:styled-content>\n 4 and\n PDE<\/jats:styled-content>\n 7 inhibition work through both common and distinct pathways. Rolipram administration caused an increase in\n IL<\/jats:styled-content>\n \u201010 and\n IL<\/jats:styled-content>\n \u201027 expression which was not found after\n TC<\/jats:styled-content>\n 3.6 treatment. On the other hand, both inhibitors reduced\n IL<\/jats:styled-content>\n \u201017 levels, prevented infiltration in\n CNS<\/jats:styled-content>\n and increased the expression of the\n T<\/jats:styled-content>\n regulator cell marker\n F<\/jats:styled-content>\n oxp3.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions and Implications<\/jats:title>\n \n These results provide new information about the effects of Rolipram on\n EAE<\/jats:styled-content>\n , underline\n PDE<\/jats:styled-content>\n 7 inhibition as a new therapeutic target for inflammatory diseases and show the value of\n TC<\/jats:styled-content>\n 3.6 to prevent\n EAE<\/jats:styled-content>\n , with possible consequences for new therapeutic tools in\n MS<\/jats:styled-content>\n .\n <\/jats:p>\n 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