{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:05:23Z","timestamp":1761581123131},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,10,28]],"date-time":"2013-10-28T00:00:00Z","timestamp":1382918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>The modulation of the spontaneous electrical and <jats:styled-content style=\"fixed-case\"><jats:roman>Ca<jats:sup>2+<\/jats:sup><\/jats:roman><\/jats:styled-content> signals underlying pyeloureteric peristalsis upon nicotinic receptor activation located on primary sensory afferents (<jats:styled-content style=\"fixed-case\">PSAs<\/jats:styled-content>) was investigated in the mouse renal pelvis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Contractile activity was followed using video microscopy, electrical and <jats:styled-content style=\"fixed-case\"><jats:roman>Ca<jats:sup>2+<\/jats:sup><\/jats:roman><\/jats:styled-content> signals in typical and atypical smooth muscle cells (<jats:styled-content style=\"fixed-case\">TSMCs<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ASMCs<\/jats:styled-content>) within the renal pelvis were recorded separately using intracellular microelectrodes and Fluo\u20104 <jats:styled-content style=\"fixed-case\"><jats:roman>Ca<jats:sup>2+<\/jats:sup><\/jats:roman><\/jats:styled-content> imaging.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Nicotine and carbachol (<jats:styled-content style=\"fixed-case\">CCh<\/jats:styled-content>; 1\u2013100\u2009\u03bcM) transiently reduced the frequency and increased the amplitude of spontaneous phasic contractions in a manner unaffected by muscarininc antagonists, 4\u2010<jats:styled-content style=\"fixed-case\">DAMP<\/jats:styled-content> (1,1\u2010dimethyl\u20104\u2010diphenylacetoxypiperidinium iodide) and pirenzipine (10\u2009nM) or L\u2010<jats:styled-content style=\"fixed-case\">NAME<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u03c9\u2010nitroarginine methyl ester; 200\u2009\u03bcM), inhibitor of <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content> synthesis, but blocked by the nicotinic antagonist, hexamethonium or capsaicin, depletor of <jats:styled-content style=\"fixed-case\">PSA<\/jats:styled-content> neuropeptides. These negative chronotropic and delayed positive inotropic effects of <jats:styled-content style=\"fixed-case\">CCh<\/jats:styled-content> on <jats:styled-content style=\"fixed-case\">TSMC<\/jats:styled-content> contractions, action potentials and <jats:styled-content style=\"fixed-case\"><jats:roman>Ca<jats:sup>2+<\/jats:sup><\/jats:roman><\/jats:styled-content> transients were inhibited by glibenclamide (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>lib; 1\u2009\u03bcM), blocker of <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>\u2010dependent <jats:styled-content style=\"fixed-case\">K<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">KATP<\/jats:styled-content>) channels. Nicotinic receptor\u2010evoked inhibition of the spontaneous <jats:styled-content style=\"fixed-case\"><jats:roman>Ca<jats:sup>2+<\/jats:sup><\/jats:roman><\/jats:styled-content> transients in <jats:styled-content style=\"fixed-case\">ASMCs<\/jats:styled-content> was prevented by capsaicin but not <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>lib. In contrast, the negative inotropic and chronotropic effects of the non\u2010selective <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content> inhibitor indomethacin were not prevented by <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>lib.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>The negative chronotropic effect of nicotinic receptor activation results from the release of calcitonin gene\u2010related peptide (<jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content>) from <jats:styled-content style=\"fixed-case\">PSAs<\/jats:styled-content>, which suppresses <jats:styled-content style=\"fixed-case\"><jats:roman>Ca<jats:sup>2+<\/jats:sup><\/jats:roman><\/jats:styled-content> signalling in <jats:styled-content style=\"fixed-case\">ASMCs<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">PSA<\/jats:styled-content>\u2010released <jats:styled-content style=\"fixed-case\">CGRP<\/jats:styled-content> also evokes a transient hyperpolarization in <jats:styled-content style=\"fixed-case\">TSMCs<\/jats:styled-content> upon the opening of <jats:styled-content style=\"fixed-case\">KATP<\/jats:styled-content> channels, which reduces contraction propagation but promotes the recruitment of <jats:styled-content style=\"fixed-case\">TSMC <jats:roman>Ca<jats:sup>2+<\/jats:sup><\/jats:roman><\/jats:styled-content> channels that underlie the delayed positive inotropic effects of <jats:styled-content style=\"fixed-case\">CCh<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.12395","type":"journal-article","created":{"date-parts":[[2013,9,4]],"date-time":"2013-09-04T14:17:35Z","timestamp":1378304255000},"page":"1221-1232","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Nicotinic receptor activation on primary sensory afferents modulates autorhythmicity in the mouse renal pelvis"],"prefix":"10.1111","volume":"170","author":[{"given":"M J","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Physiology School of Biomedical Sciences Monash University  Clayton Vic. 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