{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T17:48:41Z","timestamp":1787852921263,"version":"build-2784847793"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,12,23]],"date-time":"2013-12-23T00:00:00Z","timestamp":1387756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2014,1]]},"abstract":"Background and Purpose<\/jats:title>L<\/jats:styled-content>\u2010<\/jats:italic>DOPA<\/jats:styled-content> is generally considered to alleviate the symptoms of P<\/jats:styled-content>arkinson's disease by its conversion to dopamine. We have proposed that DOPA<\/jats:styled-content> is itself a neurotransmitter in the CNS. However, specific receptors for DOPA<\/jats:styled-content> have not been identified. Recently, the gene product of ocular albinism 1<\/jats:italic> (OA<\/jats:styled-content>1) was found to exhibit DOPA<\/jats:styled-content>\u2010binding activity. Here, we have investigated whether OA<\/jats:styled-content>1 is a functional receptor of DOPA<\/jats:styled-content> in the nucleus tractus solitarii (NTS<\/jats:styled-content>).<\/jats:p><\/jats:sec>Experimental Approach<\/jats:title>We examined immunohistochemical expression of OA<\/jats:styled-content>1 in the NTS<\/jats:styled-content>, and the effects of DOPA<\/jats:styled-content> microinjected into the depressor sites of NTS<\/jats:styled-content> on blood pressure and heart rate in anaesthetized rats, with or without prior knock\u2010down of OA<\/jats:styled-content>1 in the NTS,<\/jats:styled-content> using shRNA<\/jats:styled-content> against OA<\/jats:styled-content>1.<\/jats:p><\/jats:sec>Key Results<\/jats:title>Using a specific OA<\/jats:styled-content>1 antibody, OA<\/jats:styled-content>1\u2010positive cells and nerve fibres were found in the depressor sites of the NTS<\/jats:styled-content>. OA<\/jats:styled-content>1 expression in the NTS<\/jats:styled-content> was markedly suppressed by microinjection into the NTS<\/jats:styled-content> of adenovirus vectors carrying the relevant shRNA<\/jats:styled-content> sequences against OA<\/jats:styled-content>1. In animals treated with OA<\/jats:styled-content>1 shRNA<\/jats:styled-content>, depressor and bradycardic responses to DOPA,<\/jats:styled-content> but not those to glutamate, microinjected into the NTS<\/jats:styled-content> were blocked. Bilateral injections into the NTS<\/jats:styled-content> of DOPA<\/jats:styled-content> cyclohexyl ester, a competitive antagonist against OA<\/jats:styled-content>1, suppressed phenylephrine\u2010induced bradycardic responses without affecting blood pressure responses.<\/jats:p><\/jats:sec>Conclusion and Implications<\/jats:title>OA<\/jats:styled-content>1 acted as a functional receptor for DOPA<\/jats:styled-content> in the NTS<\/jats:styled-content>, mediating depressor and bradycardic responses. Our results add to the evidence for a central neurotransmitter role for DOPA, without conversion to dopamine.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.12459","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T02:31:09Z","timestamp":1381545069000},"page":"403-414","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["The protein Ocular albinism 1 is the orphan GPCR GPR<\/scp>143 and mediates depressor and bradycardic responses to DOPA<\/scp> in the nucleus tractus solitarii"],"prefix":"10.1111","volume":"171","author":[{"given":"Y","family":"Hiroshima","sequence":"first","affiliation":[{"name":"Department of Gastroenterological Surgery Yokohama City University Graduate School of Medicine Yokohama Japan"},{"name":"Department of Molecular Pharmacology and Neurobiology Yokohama City University Graduate School of Medicine Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Yokohama City University Graduate School of Medicine Yokohama Japan"},{"name":"Department of Molecular Pharmacology and Neurobiology Yokohama City University Graduate School of Medicine Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Neurobiology Yokohama City University Graduate School of Medicine Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Masukawa","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Neurobiology Yokohama City University Graduate School of Medicine Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Neurobiology Yokohama City University Graduate School of Medicine Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Muraoka","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Neurobiology Yokohama City University Graduate School of Medicine Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Kamiya","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Neurobiology Yokohama City University Graduate School of Medicine Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Neurobiology Yokohama City University Graduate School of Medicine Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Child Development Hoshi University School of Pharmacy and Pharmaceutical Sciences Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Matsuzaki","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine Osaka University Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Endo","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Yokohama City University Graduate School of Medicine Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Goshima","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Neurobiology Yokohama City University Graduate School of Medicine Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,12,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(99)00233-6"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12444"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/chemse\/21.3.387"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4018-11.2011"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/215852b0"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.1.5694.474"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1996.0020"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.82.40"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1980.tb02919.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1986.tb11139.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1988.tb02470.x"},{"key":"e_1_2_8_13_1","first-page":"466","article-title":"L\u2010dihydroxyphenylalanine methyl\u2010ester is a potent competitive antagonist of the L\u2010dihydroxyphenylalanine\u2010induced facilitation of the evoked release of endogenous norepinephrine from rat hypothalamic slices","volume":"258","author":"Goshima Y","year":"1991","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1975.00490430072013"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00702-011-0698-2"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00872.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(92)90090-T"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0060236"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00873.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1566-0702(01)00370-8"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00169.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(93)90082-U"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(02)00325-X"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(99)00031-4"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1976.03910040593001"},{"key":"e_1_2_8_26_1","first-page":"R1065","article-title":"Both NMDA and non\u2010NMDA receptors in the NTS participate in the baroreceptor reflex in rats","volume":"267","author":"Ohta H","year":"1994","journal-title":"Am J Physiol"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(88)90629-5"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/12715"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00008-2"},{"key":"e_1_2_8_30_1","first-page":"465","article-title":"Pharmacology of reticulospinal vasomotor neurons in cardiovascular regulation","volume":"48","author":"Sun MK","year":"1996","journal-title":"Pharmacol Rev"},{"key":"e_1_2_8_31_1","first-page":"4333","article-title":"Expression pattern of the ocular albinism type 1 (Oa1) gene in the murine retinal pigment epithelium","volume":"41","author":"Surace EM","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6105709"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpt1970115740"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(94)90321-2"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbph.12459","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bph.12459","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T19:25:05Z","timestamp":1694633105000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1111\/bph.12459"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12,23]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1111\/bph.12459"],"URL":"https:\/\/doi.org\/10.1111\/bph.12459","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,23]]},"assertion":[{"value":"2013-05-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-12-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}