{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:44:49Z","timestamp":1771634689853,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,1,13]],"date-time":"2014-01-13T00:00:00Z","timestamp":1389571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Damascus University, Syria"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2014,2]]},"abstract":"Background and Purpose<\/jats:title>TheP2Y14<\/jats:sub><\/jats:styled-content>receptor is the newest member of theP2Y<\/jats:styled-content>receptor family; it isG<\/jats:styled-content>i\/o<\/jats:sub>protein\u2010coupled and is activated byUDP<\/jats:styled-content>and selectively byUDP<\/jats:styled-content>\u2010glucose andMRS2690<\/jats:styled-content>(2\u2010thiouridine\u20105\u2032\u2010diphosphoglucose) (7\u201310\u2010fold more potent thanUDP<\/jats:styled-content>\u2010glucose). This study investigated whetherP2Y14<\/jats:sub><\/jats:styled-content>receptors were functionally expressed in porcine isolated pancreatic arteries.<\/jats:p><\/jats:sec>Experimental Approach<\/jats:title>Pancreatic arteries were prepared for isometric tension recording andUDP<\/jats:styled-content>\u2010glucose,UDP<\/jats:styled-content>andMRS2690<\/jats:styled-content>were applied cumulatively after preconstriction withU46619<\/jats:styled-content>, aTxA2<\/jats:sub><\/jats:styled-content>mimetic. Levels of phosphorylated myosin light chain 2 (MLC2<\/jats:styled-content>) were assessed withW<\/jats:styled-content>estern blotting.cAMP<\/jats:styled-content>concentrations were assessed using a competitive enzyme immunoassay kit.<\/jats:p><\/jats:sec>Key Results<\/jats:title>Concentration\u2010dependent contractions with a rank order of potency ofMRS2690<\/jats:styled-content>(10\u2010fold) >UDP<\/jats:styled-content>\u2010glucose \u2265UDP<\/jats:styled-content>were recorded. These contractions were reduced byPPTN<\/jats:styled-content>{4\u2010[4\u2010(piperidin\u20104\u2010yl)phenyl]\u20107\u2010[4\u2010(trifluoromethyl)phenyl]\u20102\u2010naphthoic acid}, a selective antagonist ofP2Y14<\/jats:sub><\/jats:styled-content>receptors, which did not affect responses to UTP. Contraction toUDP<\/jats:styled-content>\u2010glucose was not affected byMRS2578<\/jats:styled-content>, aP2Y6<\/jats:sub><\/jats:styled-content>receptor selective antagonist. RaisingcAMP<\/jats:styled-content>levels and forskolin, in the presence of U46619, enhanced contractions toUDP<\/jats:styled-content>\u2010glucose. In addition,UDP<\/jats:styled-content>\u2010glucose andMRS2690<\/jats:styled-content>inhibited forskolin\u2010stimulatedcAMP<\/jats:styled-content>levels. Removal of the endothelium and inhibition of endothelium\u2010derived contractile agents (TxA2<\/jats:sub><\/jats:styled-content>,PGF2\u03b1<\/jats:sub><\/jats:styled-content>and endothelin\u20101) inhibited contractions to UDP glucose.Y<\/jats:styled-content>\u201027632, nifedipine and thapsigargin also reduced contractions to the agonists.UDP<\/jats:styled-content>\u2010glucose andMRS2690<\/jats:styled-content>increasedMLC2<\/jats:styled-content>phosphorylation, which was blocked byPPTN<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusions and Implications<\/jats:title>P2Y14<\/jats:sub><\/jats:styled-content>receptors play a novel vasocontractile role in porcine pancreatic arteries, mediating contraction viacAMP<\/jats:styled-content>\u2010dependent mechanisms, elevation of intracellularCa2<\/jats:sup><\/jats:styled-content>+<\/jats:sup>levels, activation ofRhoA<\/jats:styled-content>\/ROCK<\/jats:styled-content>signalling andMLC2<\/jats:styled-content>, along with release ofTxA2<\/jats:sub><\/jats:styled-content>,PGF2\u03b1<\/jats:sub><\/jats:styled-content>and endothelin\u20101.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.12473","type":"journal-article","created":{"date-parts":[[2013,10,21]],"date-time":"2013-10-21T08:50:44Z","timestamp":1382345444000},"page":"701-713","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Novel vasocontractile role of theP2Y14<\/sub><\/scp>receptor: characterization of its signalling in porcine isolated pancreatic arteries"],"prefix":"10.1111","volume":"171","author":[{"given":"M","family":"Alsaqati","sequence":"first","affiliation":[{"name":"Life Sciences University of Nottingham Nottingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M L","family":"Latif","sequence":"additional","affiliation":[{"name":"Life Sciences University of Nottingham Nottingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S L F","family":"Chan","sequence":"additional","affiliation":[{"name":"Life Sciences University of Nottingham Nottingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V","family":"Ralevic","sequence":"additional","affiliation":[{"name":"Life Sciences University of Nottingham Nottingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,13]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"AbbasZ LatifL RalevicV(2011).Effects of P2Y14receptor agonists UDP\u2010glucose and MRS2690 on porcine coronary artery contractility. 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