{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T14:15:38Z","timestamp":1777472138908,"version":"3.51.4"},"reference-count":57,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2014,6,10]],"date-time":"2014-06-10T00:00:00Z","timestamp":1402358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2014,7]]},"abstract":"Background and Purpose<\/jats:title>Appetite suppression induced by amphetamine has been attributed to its inhibition of neuropeptide Y<\/jats:styled-content> (NPY<\/jats:styled-content>) neurons and activation of pro\u2010opiomelanocortin (POMC<\/jats:styled-content>) neurons in the hypothalamus. This study examined whether STAT<\/jats:styled-content>3 was involved in these actions of amphetamine.<\/jats:p><\/jats:sec>Experimental Approach<\/jats:title>Rats were given amphetamine daily for 4 days. Changes in the expression of NPY<\/jats:styled-content>, POMC<\/jats:styled-content>, melanocortin MC3<\/jats:sub> receptors, PI<\/jats:styled-content>3K<\/jats:styled-content> and STAT<\/jats:styled-content>3 in the hypothalamus were assessed by RT\u2010PCR and Western blotting. Antisense oligonucleotides to STAT3 were also used.<\/jats:p><\/jats:sec>Key Results<\/jats:title>Expression of NPY<\/jats:styled-content> decreased with a maximum effect day 2 of amphetamine treatment. Expression of POMC<\/jats:styled-content>, MC3<\/jats:sub> receptors, PI<\/jats:styled-content>3K<\/jats:styled-content> and STAT<\/jats:styled-content>3 increased with a maximum response on day 2. Moreover, phosphorylation of STAT3 and its DNA<\/jats:styled-content> binding activity increased and was expressed in a similar pattern. Infusion (i.c.v.) of STAT<\/jats:styled-content>3 antisense at 60\u2009min before amphetamine treatment, partly blocked amphetamine\u2010induced anorexia and modulated expression of NPY<\/jats:styled-content>, POMC<\/jats:styled-content>, MC3<\/jats:sub> receptors and PI<\/jats:styled-content>3K<\/jats:styled-content>, indicating the involvement of STAT<\/jats:styled-content>3 in amphetamine\u2010treated rats.<\/jats:p><\/jats:sec>Conclusions and Implications<\/jats:title>Hypothalamic PI<\/jats:styled-content>3K<\/jats:styled-content>\u2013STAT<\/jats:styled-content>3 signalling participated in the regulation of NPY<\/jats:styled-content>\u2010 and POMC<\/jats:styled-content>\u2010mediated appetite suppression. These findings may contribute to a better understanding of anorectic drugs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.12667","type":"journal-article","created":{"date-parts":[[2014,3,6]],"date-time":"2014-03-06T07:41:40Z","timestamp":1394091700000},"page":"3223-3233","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Involvement of hypothalamic PI<\/scp>3K<\/scp>\u2013STAT<\/scp>3 signalling in regulating appetite suppression mediated by amphetamine"],"prefix":"10.1111","volume":"171","author":[{"given":"Shu\u2010Chen","family":"Chu","sequence":"first","affiliation":[{"name":"Department of Food Science Central Taiwan University of Science and Technology Taichung City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei\u2010Ni","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Biotechnology Chung Shan Medical University and Chung Shan Medical 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