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We used a<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced<jats:styled-content style=\"fixed-case\">ALI<\/jats:styled-content>model to determine whether<jats:styled-content style=\"fixed-case\">MaR1<\/jats:styled-content>can mitigate lung injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Male<jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice were injected, intratracheally, with either<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>(3\u2009mg\u00b7kg<jats:sup>\u22121<\/jats:sup>) or normal saline (1.5\u2009mL\u00b7kg<jats:sup>\u22121<\/jats:sup>). After this, normal saline, a low dose of<jats:styled-content style=\"fixed-case\">MaR1<\/jats:styled-content>(0.1\u2009ng per mouse) or a high dose of<jats:styled-content style=\"fixed-case\">MaR1<\/jats:styled-content>(1\u2009ng per mouse) was given i.v. Lung injury was evaluated by detecting arterial blood gas, pathohistological examination, pulmonary oedema, inflammatory cell infiltration, inflammatory cytokines in the bronchoalveolar lavage fluid and neutrophil\u2013platelet interactions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>The high dose of<jats:styled-content style=\"fixed-case\">MaR1<\/jats:styled-content>significantly inhibited<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced<jats:styled-content style=\"fixed-case\">ALI<\/jats:styled-content>by restoring oxygenation, attenuating pulmonary oedema and mitigating pathohistological changes. A combination of<jats:sc>elisa<\/jats:sc>and immunohistochemistry showed that high\u2010dose<jats:styled-content style=\"fixed-case\">MaR1<\/jats:styled-content>attenuated<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced increases in pro\u2010inflammatory cytokines (<jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1,<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 and<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106), chemokines [keratinocyte chemokine, monocyte chemoattractant protein\u20105, macrophage inflammatory protein (<jats:styled-content style=\"fixed-case\">MIP<\/jats:styled-content>)\u20101\u03b1 and<jats:styled-content style=\"fixed-case\">MIP<\/jats:styled-content>\u20101\u03b3], pulmonary myeloperoxidase activity and neutrophil infiltration in the lung tissues. Consistent with these observations, flow cytometry and Western blotting indicated that<jats:styled-content style=\"fixed-case\">MaR1<\/jats:styled-content>down\u2010regulated<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced neutrophil adhesions and suppressed the expression of intercellular adhesion molecule (<jats:styled-content style=\"fixed-case\">ICAM<\/jats:styled-content>)\u20101,<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>\u2010selection and<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>24.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>High\u2010dose<jats:styled-content style=\"fixed-case\">MaR1<\/jats:styled-content>mitigated<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced lung injury in mice by inhibiting neutrophil adhesions and decreasing the levels of pro\u2010inflammatory cytokines.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.12714","type":"journal-article","created":{"date-parts":[[2014,4,4]],"date-time":"2014-04-04T07:13:29Z","timestamp":1396595609000},"page":"3539-3550","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["<scp>M<\/scp>aresin 1 mitigates<scp>LPS<\/scp>\u2010induced acute lung injury in mice"],"prefix":"10.1111","volume":"171","author":[{"given":"Jie","family":"Gong","sequence":"first","affiliation":[{"name":"Department of Critical Care Medicine Institute of Anesthesia and Critical Care Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou\u2010yang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine Institute of Anesthesia and Critical Care Union Hospital Tongji Medical College Huazhong University of 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