{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T02:34:56Z","timestamp":1789698896487,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2014,6,25]],"date-time":"2014-06-25T00:00:00Z","timestamp":1403654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2014,7]]},"abstract":"Background and Purpose<\/jats:title>Methyl salicylate 2\u2010O<\/jats:italic><\/jats:styled-content>\u2010\u03b2\u2010d<\/jats:sc>\u2010lactoside (MSL<\/jats:styled-content>), whose chemical structure is similar to that of salicylic acid, is a natural product derivative isolated from a traditional Chinese herb. The aim of this study was to investigate the therapeutic effect of MSL<\/jats:styled-content> in mice with collagen\u2010induced arthritis (CIA<\/jats:styled-content>) and explore its underlying mechanism.<\/jats:p><\/jats:sec>Experimental Approach<\/jats:title>The anti\u2010arthritic effects of MSL<\/jats:styled-content> were evaluated on human rheumatoid fibroblast\u2010like synoviocytes (FLS<\/jats:styled-content>) in vitro<\/jats:italic> and CIA<\/jats:styled-content> in mice in vivo<\/jats:italic> by obtaining clinical scores, measuring hind paw thickness and inflammatory cytokine levels, radiographic evaluations and histopathological assessments.<\/jats:p><\/jats:sec>Key Results<\/jats:title>Treatment with MSL<\/jats:styled-content> after the onset of arthritis significantly prevented the progression and development of rheumatoid arthritis (RA<\/jats:styled-content>) in CIA<\/jats:styled-content> mice without megascopic gastric mucosa damage. In addition, MSL<\/jats:styled-content> inhibited the production of pro\u2010inflammatory mediators, the phosphorylation and translocation of NF<\/jats:styled-content>\u2010\u03baB,<\/jats:styled-content> and cell proliferation induced by TNF<\/jats:styled-content>\u2010\u03b1 in FLS<\/jats:styled-content>. MSL<\/jats:styled-content> non\u2010selectively inhibited the activity of COX<\/jats:styled-content>\u2005in vitro,<\/jats:italic> but was a more potent inhibitor of COX\u20102 than COX\u20101. MSL<\/jats:styled-content> also inhibited the phosphorylation of inhibitor of NF<\/jats:styled-content>\u2010\u03baB<\/jats:styled-content> kinase, I<\/jats:styled-content>\u03baB<\/jats:styled-content>\u03b1 and p65, thus blocking the nuclear translocation of NF<\/jats:styled-content>\u2010\u03baB<\/jats:styled-content> in TNF<\/jats:styled-content>\u2010\u03b1\u2010stimulated FLS<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusion and Implications<\/jats:title>MSL<\/jats:styled-content> exerts therapeutic effects on CIA<\/jats:styled-content> mice, suppressing the inflammatory response and joint destruction by non\u2010selectively inhibiting the activity of COX and suppressing activation of the NF\u2010\u03baB signalling pathway, but without damaging the gastric mucosa. Therefore, MSL<\/jats:styled-content> has great potential to be developed into a novel therapeutic agent for the treatment of RA<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.12715","type":"journal-article","created":{"date-parts":[[2014,4,8]],"date-time":"2014-04-08T11:11:42Z","timestamp":1396955502000},"page":"3526-3538","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Methyl salicylate lactoside inhibits inflammatory response of fibroblast\u2010like synoviocytes and joint destruction in collagen\u2010induced arthritis in mice"],"prefix":"10.1111","volume":"171","author":[{"given":"Wenyu","family":"Xin","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences & Peking Union Medical College Beijing China"},{"name":"Key Laboratory of Molecular Pharmacology and Drug 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