{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:01:27Z","timestamp":1789729287089,"version":"4.0.1"},"reference-count":50,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,2,27]],"date-time":"2015-02-27T00:00:00Z","timestamp":1424995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R37 DK54824"],"award-info":[{"award-number":["R37 DK54824"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 DK57284"],"award-info":[{"award-number":["R01 DK57284"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2015,4]]},"abstract":"\n Background and Purpose<\/jats:title>\n \n The urinary bladder urothelium expresses various receptors and in response to chemical and mechanical stimuli releases mediators, thereby modulating bladder sensory pathways. Transient receptor potential vanilloid 1 (\n TRPV<\/jats:styled-content>\n 1) ion channels and nerve growth factor (\n NGF<\/jats:styled-content>\n ) in those cells are implicated in this modulatory effect and play a role in sensitizing pain\u2010related afferent pathways during inflammation. In this study, we investigated the interaction between\n NGF<\/jats:styled-content>\n and\n TRPV<\/jats:styled-content>\n 1 channels in urothelial cells.\n <\/jats:p>\n <\/jats:sec>\n \n Experimental Approach<\/jats:title>\n \n Urothelial cells from female\n S<\/jats:styled-content>\n prague\u2010\n D<\/jats:styled-content>\n awley rat bladders were cultured to quantify membrane expression of\n TRPV<\/jats:styled-content>\n 1 channels and capsaicin\u2010induced\n ATP<\/jats:styled-content>\n release in the presence of\n NGF<\/jats:styled-content>\n alone or with\n TrKA<\/jats:styled-content>\n or PI3K inhibitors. Pain scores from rats with cyclophosphamide (\n CYP<\/jats:styled-content>\n )\u2010induced bladder inflammation were assessed after treatment with a\n TrkA<\/jats:styled-content>\n antagonist. Bladders (from control and\n CYP<\/jats:styled-content>\n rats) were collected and analysed for\n NGF<\/jats:styled-content>\n content and\n TRPV<\/jats:styled-content>\n 1 channel expression.\n <\/jats:p>\n <\/jats:sec>\n \n Key Results<\/jats:title>\n \n Cultured cells responded to\n NGF<\/jats:styled-content>\n with increased\n TRPV<\/jats:styled-content>\n 1 channel expression in the cell membrane and increased release of\n ATP<\/jats:styled-content>\n . Both responses were blocked by either a\n TrkA<\/jats:styled-content>\n antagonist or a\n PI<\/jats:styled-content>\n 3K inhibitor. Treatment\n in vivo<\/jats:italic>\n with the\n TrkA<\/jats:styled-content>\n antagonist alleviated pain symptoms and reduced\n CYP<\/jats:styled-content>\n \u2010induced\n NGF<\/jats:styled-content>\n overexpression in the mucosa. Furthermore, in urothelial cells from animals with bladder inflammation, expression of\n TRPV<\/jats:styled-content>\n 1 channels in the membrane was significantly increased.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions and Implications<\/jats:title>\n \n During bladder inflammation, increased production of\n NGF<\/jats:styled-content>\n in urothelial cells induced increased expression and activity of\n TRPV<\/jats:styled-content>\n 1 channels in the cell membrane. This effect was primarily mediated by the PI\n 3K<\/jats:styled-content>\n pathway.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/bph.12958","type":"journal-article","created":{"date-parts":[[2014,10,8]],"date-time":"2014-10-08T22:37:44Z","timestamp":1412807864000},"page":"1691-1699","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Urinary bladder inflammation induces changes in urothelial nerve growth factor and\n TRPV<\/scp>\n 1 channels"],"prefix":"10.1111","volume":"172","author":[{"given":"A","family":"Coelho","sequence":"first","affiliation":[{"name":"Department of Experimental Biology Faculty of Medicine University of Porto Porto Portugal"},{"name":"Institute for Molecular and Cell Biology (IBMC) University of Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A S","family":"Wolf\u2010Johnston","sequence":"additional","affiliation":[{"name":"Departmentof Urology Hospital S. 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