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Stability study of <jats:styled-content style=\"fixed-case\">CopNEC\u2010AmB<\/jats:styled-content> was carried out in simulated gastric fluid and simulated intestinal fluid. The <jats:styled-content style=\"fixed-case\">CopNEC\u2010AmB<\/jats:styled-content> and plain <jats:styled-content style=\"fixed-case\">AmB<\/jats:styled-content> were compared as regards their <jats:italic>in vitro<\/jats:italic> antileishmanial activity, pharmacokinetics, organ distribution and toxicity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>The optimal <jats:styled-content style=\"fixed-case\">CopNEC\u2010AmB<\/jats:styled-content> had a small globule size, low polydispersity index, high \u03b6 potential and encapsulation efficiency. The high resolution transmission electron microscopy illustrated spherical particle geometry with homogeny in their sizes. The optimal <jats:styled-content style=\"fixed-case\">CopNEC\u2010AmB<\/jats:styled-content> was found to be stable in gastrointestinal fluids showing insignificant changes in globule size and encapsulation efficiency. The <jats:styled-content style=\"fixed-case\">AUC<\/jats:styled-content><jats:sub>0\u201348<\/jats:sub> value of <jats:styled-content style=\"fixed-case\">CopNEC\u2010AmB<\/jats:styled-content> in rats was significantly improved showing 7.2\u2010fold higher oral bioavailability than free drug. The <jats:italic>in vitro<\/jats:italic> antileishmanial activity of <jats:styled-content style=\"fixed-case\">CopNEC\u2010AmB<\/jats:styled-content> was significantly higher than that of the free drug as <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>op synergistically enhanced the antileishmanial effect of AmB by causing drastic changes in the morphology of <jats:styled-content style=\"fixed-case\"><jats:italic>L<\/jats:italic><\/jats:styled-content><jats:italic>eishmania<\/jats:italic> parasite and rupturing its plasma membrane. The <jats:styled-content style=\"fixed-case\">CopNEC\u2010AmB<\/jats:styled-content> showed significantly less haemolytic toxicity and cytotoxicity and did not change the histopathology of kidney tissues as compared with AmB alone.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>This prototype <jats:styled-content style=\"fixed-case\">CopNEC<\/jats:styled-content> formulation showed improved bioavailability and had a non\u2010toxic synergistic effect on the antileishmanial activity of <jats:styled-content style=\"fixed-case\">AmB<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.13149","type":"journal-article","created":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T14:34:43Z","timestamp":1427898883000},"page":"3596-3610","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Synergistic enhancement of parasiticidal activity of amphotericin <scp>B<\/scp> 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