{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:35:22Z","timestamp":1785803722392,"version":"3.56.0"},"reference-count":74,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2015,5,15]],"date-time":"2015-05-15T00:00:00Z","timestamp":1431648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Science and Technological Development of the Republic of Serbia","award":["173014"],"award-info":[{"award-number":["173014"]}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2015,7]]},"abstract":"Background and Purpose<\/jats:title>Hydrogen sulphide reduces uterine contractility and is of potential interest as a treatment for uterine disorders. The aim of this study was to explore the mechanism of sodium sulphide (Na2<\/jats:sub>S<\/jats:roman><\/jats:styled-content>)\u2010induced relaxation of rat uterus, investigate the importance of redox effects and ion channel\u2010mediated mechanisms, and any interactions between these two mechanisms.<\/jats:p><\/jats:sec>Experimental Approach<\/jats:title>Organ bath studies were employed to assess the pharmacological effects of Na2<\/jats:sub>S<\/jats:roman><\/jats:styled-content> in uterine strips by exposing them to Na2<\/jats:sub>S<\/jats:roman><\/jats:styled-content> with or without Cl\u2212<\/jats:sup><\/jats:roman><\/jats:styled-content> channel blockers (DIDS<\/jats:styled-content>, NFA<\/jats:styled-content>, IAA<\/jats:styled-content>\u201094, T<\/jats:styled-content>16A<\/jats:styled-content>inh\u2010A<\/jats:styled-content>01, TA<\/jats:styled-content>), raised KCl<\/jats:roman><\/jats:styled-content> (15 and 75\u2009mM), K+<\/jats:sup><\/jats:roman><\/jats:styled-content> channel inhibitors (glibenclamide, TEA<\/jats:styled-content>, 4\u2010AP<\/jats:styled-content>), L<\/jats:styled-content>\u2010type Ca2+<\/jats:sup><\/jats:roman><\/jats:styled-content> channel activator (S<\/jats:styled-content>\u2010B<\/jats:styled-content>ay K<\/jats:styled-content> 8644), propranolol and methylene blue. The activities of antioxidant enzymes were measured in homogenates of treated uteri. The expression of bestrophin channel 1 (BEST<\/jats:styled-content>\u20101) was determined by Western blotting and RT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec>Key Results<\/jats:title>Na2<\/jats:sub>S<\/jats:roman><\/jats:styled-content> caused concentration\u2010dependent reversible relaxation of spontaneously active and calcium\u2010treated uteri, affecting both amplitude and frequency of contractions. Uteri exposed to 75\u2009mM KCl<\/jats:roman><\/jats:styled-content> were less sensitive to Na2<\/jats:sub>S<\/jats:roman><\/jats:styled-content> compared with uteri in 15\u2009mM KCl<\/jats:roman><\/jats:styled-content>. Na2<\/jats:sub>S<\/jats:roman><\/jats:styled-content>\u2010induced relaxations were abolished by DIDS, but unaffected by other modulators or by the absence of extracellular HCO3<\/jats:sub>\u2212<\/jats:sup><\/jats:roman><\/jats:styled-content>, suggesting the involvement of chloride ion channels. Na2<\/jats:sub>S<\/jats:roman><\/jats:styled-content> in combination with different modulators provoked specific changes in the anti\u2010oxidant profiles of uteri. The expression of BEST<\/jats:styled-content>\u20101, both mRNA<\/jats:styled-content> and protein, was demonstrated in rat uteri.<\/jats:p><\/jats:sec>Conclusions and Implications<\/jats:title>The relaxant effects of Na2<\/jats:sub>S<\/jats:roman><\/jats:styled-content> in rat uteri are mediated mainly via a DIDS<\/jats:styled-content>\u2010sensitive Cl\u2212<\/jats:sup><\/jats:roman><\/jats:styled-content>\u2010pathway. Components of the relaxation are redox\u2010 and Ca2+<\/jats:sup><\/jats:roman><\/jats:styled-content>\u2010dependent.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.13161","type":"journal-article","created":{"date-parts":[[2015,4,8]],"date-time":"2015-04-08T10:06:45Z","timestamp":1428487605000},"page":"3671-3686","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Chloride channels mediate sodium sulphide\u2010induced relaxation in rat uteri"],"prefix":"10.1111","volume":"172","author":[{"given":"Ana","family":"Miju\u0161kovi\u0107","sequence":"first","affiliation":[{"name":"Department of Physiology Institute for Biological Research \u2018Sini\u0161a Stankovi\u0107\u2032 University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra Nikoli\u0107","family":"Koki\u0107","sequence":"additional","affiliation":[{"name":"Department of Physiology Institute for Biological Research \u2018Sini\u0161a Stankovi\u0107\u2032 University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zorana Ore\u0161\u010danin","family":"Du\u0161i\u0107","sequence":"additional","affiliation":[{"name":"Department of Physiology Institute for Biological Research \u2018Sini\u0161a Stankovi\u0107\u2032 University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marija","family":"Slavi\u0107","sequence":"additional","affiliation":[{"name":"Department of Physiology Institute for Biological Research \u2018Sini\u0161a Stankovi\u0107\u2032 University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihajlo B","family":"Spasi\u0107","sequence":"additional","affiliation":[{"name":"Department of Physiology Institute for Biological Research \u2018Sini\u0161a Stankovi\u0107\u2032 University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Du\u0161ko","family":"Blagojevi\u0107","sequence":"additional","affiliation":[{"name":"Department of Physiology Institute for Biological Research \u2018Sini\u0161a Stankovi\u0107\u2032 University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706644"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI927"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmq016"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12445"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12447"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12450"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12451"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00005192"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00490.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2014.12.008"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2014.06.018"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvr111"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704226"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1163518"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2005.tb00366.x"},{"key":"e_1_2_10_17_1","first-page":"283","volume-title":"Handbook of Methods for Oxygen Radical Research","author":"Claiborne A","year":"1985"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9910185"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2009.01.011"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.02376"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.033506"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-006-0062-x"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm3012036"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01921531"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.186478"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.5041"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2013.05.029"},{"key":"e_1_2_10_28_1","volume-title":"Applied Statistics for Behavioral Sciences","author":"Hinkle ED","year":"1994"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-003-1224-7"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2012.11.001"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00872.x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-007-0245-z"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1139\/y05-040"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.05.063"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2012.06.003"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0089083"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2013.07.001"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424\u2010014\u20101572\u20105"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.4149\/gpb_2009_02_190"},{"key":"e_1_2_10_41_1","volume-title":"Multivariate Statistical Methods","author":"Manley BFJ","year":"1986"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1519-69842002000400008"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00873.x"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharep.2013.12.012"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019364"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)45228-9"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.5108"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2000464"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.240242"},{"key":"e_1_2_10_50_1","first-page":"74","article-title":"Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase","volume":"70","author":"Paglia DE","year":"1967","journal-title":"J Lab Clin Med"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-7-10"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr300163e"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1143"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00039.2011"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.4612"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00398.2007"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12084"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2010.08.001"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046278"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2011.058248"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2011.058198"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1995.1228"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0773.2001.880407.x"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.277152"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.071258"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-3209-8_15"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-012-0779-2"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.052692999"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.105.017467"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.2010.05351.x"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00729.2010"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2012.02187.x"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12431"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037073"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.21.6008"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbph.13161","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bph.13161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T09:26:03Z","timestamp":1694510763000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1111\/bph.13161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,15]]},"references-count":74,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2015,7]]}},"alternative-id":["10.1111\/bph.13161"],"URL":"https:\/\/doi.org\/10.1111\/bph.13161","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,15]]},"assertion":[{"value":"2014-07-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-03-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-05-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}