{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T12:21:51Z","timestamp":1765887711587,"version":"3.41.0"},"reference-count":49,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2015,10,14]],"date-time":"2015-10-14T00:00:00Z","timestamp":1444780800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL094447, HL027339"],"award-info":[{"award-number":["HL094447, HL027339"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>Stimulation of the A<jats:sub>1<\/jats:sub>adenosine receptor and angiotensin II receptor type\u20101 (AT<jats:sub>1<\/jats:sub>receptor) causes vasoconstriction through activation of cytochrome P450 4A (CYP4A) and ERK1\/2. Thus, we hypothesized that acute angiotensin II activation alters the vasomotor response induced by the non\u2010selective adenosine receptor agonist, NECA, in mouse mesenteric arteries (MAs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>We used a Danish Myo Technology wire myograph to measure muscle tension in isolated MAs from wild type (WT), A<jats:sub>1<\/jats:sub>receptor and A<jats:sub>2B<\/jats:sub>receptor knockout (KO) mice. Western blots were performed to determine the expression of AT<jats:sub>1<\/jats:sub>receptors and CYP4A.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Acute exposure (15\u2009min) to angiotensin II attenuated the NECA\u2010dependent vasodilatation and enhanced vasoconstriction. This vasoconstrictor effect of angiotensin II in NECA\u2010treated MAs was abolished in A<jats:sub>1<\/jats:sub>receptor KO mice and in WT mice treated with the A<jats:sub>1<\/jats:sub>receptor antagonist DPCPX, CYP4A inhibitor HET0016 and ERK1\/2 inhibitor PD98059. In MAs from A<jats:sub>2B<\/jats:sub>receptor KO mice, the vasoconstrictor effect of angiotensin II on the NECA\u2010induced response was shown to be dependent on A<jats:sub>1<\/jats:sub>receptors. Furthermore, in A<jats:sub>2B<\/jats:sub>receptor KO mice, the expression of AT<jats:sub>1<\/jats:sub>receptors and CYP4A was increased and the angiotensin II\u2010induced vasoconstriction enhanced. In addition, inhibition of K<jats:sub>ATP<\/jats:sub>channels with glibenclamide significantly reduced NECA\u2010induced vasodilatation in WT mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>Acute angiotensin II stimulation enhanced A<jats:sub>1<\/jats:sub>receptor\u2010dependent vasoconstriction and inhibited A<jats:sub>2B<\/jats:sub>receptor\u2010dependent vasodilatation, leading to a net vasoconstriction and altered vasomotor response to NECA in MAs. This interaction may be important in the regulation of BP.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.13265","type":"journal-article","created":{"date-parts":[[2015,7,31]],"date-time":"2015-07-31T02:22:53Z","timestamp":1438309373000},"page":"4959-4969","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Angiotensin II stimulation alters vasomotor response to adenosine in mouse mesenteric artery: role for A<sub>1<\/sub>and A<sub>2B<\/sub>adenosine receptors"],"prefix":"10.1111","volume":"172","author":[{"given":"Vishal R","family":"Yadav","sequence":"first","affiliation":[{"name":"Department of Physiology and Pharmacology School of Medicine Morgantown WV USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed A","family":"Nayeem","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, School of Pharmacy West Virginia University Morgantown WV 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