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In this study we evaluated the influence of K<jats:sub>v<\/jats:sub>7 channel activation on smooth muscle relaxation in rat penile arteries and corpus cavernosum from normal and spontaneously hypertensive, heart failure\u2010prone (SHHF) rats \u2013 a rat model of human metabolic syndrome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Quantitative PCR and immunohistochemistry were used to determine the expression of <jats:italic>KCNQ<\/jats:italic> isoforms in penile tissue. Isometric tension was measured in intracavernous arterial rings and corpus cavernosum strips isolated from normal and SHHF rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Transcripts for <jats:italic>KCNQ3<\/jats:italic>, <jats:italic>KCNQ4<\/jats:italic> and <jats:italic>KCNQ5<\/jats:italic> were detected in penile arteries and corpus cavernosum. <jats:italic>KCNQ1<\/jats:italic> was only found in corpus cavernosum. Immunofluorescence signals to K<jats:sub>v<\/jats:sub>7.4 and K<jats:sub>v<\/jats:sub>7.5 were found in penile arteries, penile veins and corpus cavernosum. The K<jats:sub>v<\/jats:sub>7.2\u20137.5 activators, ML213 and BMS204352, relaxed pre\u2010contracted penile arteries and corpus cavernosum independently of nitric oxide synthase or endothelium\u2010derived hyperpolarization. Relaxations to sildenafil, a PDE5 inhibitor, and sodium nitroprusside (SNP), an nitric oxide donor, were reduced by blocking K<jats:sub>v<\/jats:sub>7 channels with linopirdine in penile arteries and corpus cavernosum. In SHHF rat penile arteries and corpus cavernosum, relaxations to ML213 and BMS204352 were attenuated, and the blocking effect of linopirdine on sildenafil\u2010induced and SNP\u2010induced relaxations reduced. <jats:italic>KCNQ3<\/jats:italic>, <jats:italic>KCNQ4<\/jats:italic> and <jats:italic>KCNQ5<\/jats:italic> were down\u2010regulated, and <jats:italic>KCNQ1<\/jats:italic> was up\u2010regulated in corpus cavernosum from SHHF rats. <jats:italic>KCNQ1\u20135<\/jats:italic> transcripts remained unchanged in penile arteries from SHHF rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>These data suggest that K<jats:sub>v<\/jats:sub>7 channels play a role in erectile function and contribute to the pathophysiology of erectile dysfunction, an early indicator of cardiovascular disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.13444","type":"journal-article","created":{"date-parts":[[2016,1,23]],"date-time":"2016-01-23T18:48:44Z","timestamp":1453574924000},"page":"1478-1490","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Molecular and functional characterization of K<sub>v<\/sub>7 channels in penile arteries and corpus cavernosum of healthy and metabolic syndrome rats"],"prefix":"10.1111","volume":"173","author":[{"given":"T A","family":"Jepps","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S P","family":"Olesen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen 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