{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T01:38:47Z","timestamp":1768873127149,"version":"3.49.0"},"reference-count":56,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,4,6]],"date-time":"2016-04-06T00:00:00Z","timestamp":1459900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>Fab fragments (Fabs) of antibodies have the ability to bind to specific allergens but lack the Fc portion that exerts effector functions via binding to receptors including Fc\u03b5R1 on mast cells. In the present study, we investigated whether intranasal administration of the effector function\u2010lacking Fabs of a monoclonal antibody IgG1 (mAb, P1\u20108) to the major allergen Cry j1 of Japanese cedar pollen (JCP) suppressed JCP\u2010induced allergic rhinitis in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Balb\/c mice sensitized with JCP on days 0 and 14 were challenged intranasally with the pollen on days 28, 29, 30 and 35. Fabs prepared by the digestion of P1\u20108 with papain were also administered intranasally 15\u00a0min before each JCP challenge.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Intranasal administration of P1\u20108 Fabs was followed by marked suppression of sneezing and nasal rubbing in mice with JCP\u2010induced allergic rhinitis. The suppression of these allergic symptoms by P1\u20108 Fabs was associated with decreases in mast cells and eosinophils and decreased hyperplasia of goblet cells in the nasal mucosa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>These results demonstrated that intranasal exposure to P1\u20108 Fabs was effective in suppressing JCP\u2010induced allergic rhinitis in mice, suggesting that allergen\u2010specific mAb Fabs might be used as a tool to regulate allergic pollinosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.13463","type":"journal-article","created":{"date-parts":[[2016,2,20]],"date-time":"2016-02-20T15:43:22Z","timestamp":1455983002000},"page":"1629-1638","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Intranasal exposure to monoclonal antibody Fab fragments to Japanese cedar pollen Cry j1 suppresses Japanese cedar pollen\u2010induced allergic rhinitis"],"prefix":"10.1111","volume":"173","author":[{"given":"S","family":"Yoshino","sequence":"first","affiliation":[{"name":"Department of Pharmacology Kobe Pharmaceutical University  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Mizutani","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Kobe Pharmaceutical University  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,6]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"969","article-title":"Is allergen immunotherapy effective in asthma? 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