{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:37:05Z","timestamp":1790516225437,"version":"4.1.0"},"reference-count":67,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2016,10,23]],"date-time":"2016-10-23T00:00:00Z","timestamp":1477180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>Perivascular adipose tissue (PVAT) surrounds most blood vessels and secretes numerous active substances, including adiponectin, which produce a net anticontractile effect in healthy individuals. AMPK is a key mediator of cellular energy balance and may mediate the vascular effects of adiponectin. In this study, we investigated the role of AMPK within PVAT in mediating the anticontractile effect of PVAT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Endothelium\u2010denuded aortic rings from wild\u2010type (WT; Sv129) and \u03b1<jats:sub>1<\/jats:sub>AMPK knockout (KO) mice were mounted on a wire myograph. Dose\u2013response curves to the AMPK\u2010independent vasodilator cromakalim were studied in vessels with and without PVAT, and effect of pre\u2010incubation with conditioned media and adiponectin on relaxation was also studied. The effect of AMPK\u03b11 KO on the secretory profile of PVAT was assessed by <jats:sc>elisa<\/jats:sc>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Thoracic aortic PVAT from KO mice was morphologically indistinct from that of WT and primarily composed of brown adipose tissue. PVAT augmented relaxation to cromakalim in WT but not KO aortic rings. Addition of WT PVAT augmented relaxation in KO aortic rings but KO PVAT had no effect in WT rings. PVAT from KO mice secreted significantly less adiponectin and addition of adiponectin to either KO or WT aortic rings without PVAT augmented relaxation to cromakalim. An adiponectin blocking peptide significantly attenuated relaxation in WT rings with PVAT but not in KO rings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>AMPK\u03b11 has a critical role in maintaining the anticontractile actions of PVAT; an effect independent of the endothelium but likely mediated through altered adiponectin secretion or sensitivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Linked Articles<\/jats:title><jats:p>This article is part of a themed section on Molecular Mechanisms Regulating Perivascular Adipose Tissue \u2013 Potential Pharmacological Targets? To view the other articles in this section visit <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bph.v174.20\/issuetoc\">http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bph.v174.20\/issuetoc<\/jats:ext-link><\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.13633","type":"journal-article","created":{"date-parts":[[2016,9,26]],"date-time":"2016-09-26T18:36:44Z","timestamp":1474915004000},"page":"3398-3410","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Deletion of AMPK\u03b11 attenuates the anticontractile effect of perivascular adipose tissue (PVAT) and reduces adiponectin release"],"prefix":"10.1111","volume":"174","author":[{"given":"Tarek A M","family":"Almabrouk","sequence":"first","affiliation":[{"name":"Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary and Life Sciences University of Glasgow  Glasgow 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