{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T22:08:14Z","timestamp":1791065294640,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,11,28]],"date-time":"2016-11-28T00:00:00Z","timestamp":1480291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["2622\u201014\u20109"],"award-info":[{"award-number":["2622\u201014\u20109"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>Peptides from venomous animals have long been important for understanding pain mechanisms and for the discovery of pain treatments. Here, we hypothesized that Ph\u03b11\u03b2, a peptide from the venom of the armed spider <jats:styled-content style=\"fixed-case\"><jats:italic>Phoneutria nigriventer<\/jats:italic><\/jats:styled-content>, produces analgesia by blocking the TRPA1 channel.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Cultured rat dorsal root ganglion (DRG) neurons, human fetal lung fibroblasts (IMR90) or HEK293 cells expressing the human TRPA1 (hTRPA1\u2010HEK293), human TRPV1 (hTRPV1\u2010HEK293) or human TRPV4 channels (hTRPV4\u2010HEK293), were used for calcium imaging and electrophysiology. Nociceptive responses induced by TRPA1, TRPV1 or TRPV4 agonists or by bortezomib were investigated in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Ph\u03b11\u03b2 selectively inhibited calcium responses and currents evoked by the TRPA1 agonist, allyl isothiocyanate (AITC), on hTRPA1\u2010HEK293, IMR90 fibroblasts and DRG neurons. Ph\u03b11\u03b2 did not affect calcium responses evoked by selective TRPV1 (capsaicin) or TRPV4 (GSK 1016790A) agonists on the various cell types. Intrathecal (i.t.) and intraplantar (i.pl.) administration of low doses of Ph\u03b11\u03b2 (up to 300\u00a0pmol per paw) attenuated acute nociception and mechanical and cold hyperalgesia evoked by AITC (i.t. or i.pl.), without affecting responses produced by capsaicin or hypotonic solution. Notably, Ph\u03b11\u03b2 abated the TRPA1\u2010dependent neuropathic pain\u2010like responses induced by bortezomib. <jats:italic>In vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> inhibition of TRPA1 by Ph\u03b11\u03b2 was reproduced by a recombinant form of the peptide, CTK 01512\u20102.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>Ph\u03b11\u03b2 and CTK 01512\u20132 selectively target TRPA1, but not other TRP channels. This specific action underlines the potential of Ph\u03b11\u03b2 and CTK 01512\u20102 for pain treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.13652","type":"journal-article","created":{"date-parts":[[2016,10,19]],"date-time":"2016-10-19T12:32:40Z","timestamp":1476880360000},"page":"57-69","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":92,"title":["The peptide Ph\u03b11\u03b2, from spider venom, acts as a TRPA1 channel antagonist with antinociceptive effects in mice"],"prefix":"10.1111","volume":"174","author":[{"given":"Raquel","family":"Tonello","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas: Bioqu\u00edmica Toxicol\u00f3gica Universidade Federal de Santa Maria  Santa Maria Brazil"},{"name":"Departmento de Farmacologia Universidade Federal de Santa Catarina  Florian\u00f3polis 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