{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T03:43:17Z","timestamp":1774323797405,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,12,20]],"date-time":"2016-12-20T00:00:00Z","timestamp":1482192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>K<jats:sub>V<\/jats:sub>7.1 voltage\u2010gated potassium channels are expressed in vascular smooth muscle cells (VSMC) of diverse arteries, including mesenteric arteries. Based on pharmacological evidence using R\u2010L3 (K<jats:sub>V<\/jats:sub>7.1 channel opener), HMR1556, chromanol 293B (K<jats:sub>V<\/jats:sub>7.1 channel blockers), stimulation of these channels has been suggested to evoke profound relaxation in various vascular beds of rats. However, the specificity of these drugs<jats:italic>in vivo<\/jats:italic>is uncertain.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>We used<jats:italic>Kcnq1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>mice and pharmacological tools to determine whether K<jats:sub>V<\/jats:sub>7.1 channels play a role in the regulation of arterial tone.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>R\u2010L3 produced similar concentration\u2010dependent relaxations (EC<jats:sub>50<\/jats:sub>\u00a0~\u00a01.4\u00a0\u03bcM) of arteries from wild\u2010type (<jats:italic>Kcnq1<\/jats:italic><jats:sup>+\/+<\/jats:sup>) and<jats:italic>Kcnq1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>mice, pre\u2010contracted with either phenylephrine or 60\u00a0mM KCl. This relaxation was not affected by 10\u00a0\u03bcM chromanol 293B, 10\u00a0\u03bcM HMR1556 or 30\u00a0\u03bcM XE991 (pan\u2010K<jats:sub>V<\/jats:sub>7 channel blocker). The anti\u2010contractile effects of the perivascular adipose tissue (PVAT) were normal in<jats:italic>Kcnq1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>arteries. Chromanol 293B and HMR1556 did not affect the anti\u2010contractile effects of (PVAT). Isolated VSMCs from<jats:italic>Kcnq1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>mice exhibited normal peak K<jats:sub>V<\/jats:sub>currents. The K<jats:sub>V<\/jats:sub>7.2\u20135 channel opener retigabine caused similar relaxations in<jats:italic>Kcnq1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>and wild\u2010type vessels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Implications<\/jats:title><jats:p>We conclude that K<jats:sub>V<\/jats:sub>7.1 channels were apparently not involved in the control of arterial tone by \u03b1<jats:sub>1<\/jats:sub>\u2010adrenoceptor agonists and PVAT. In addition, R\u2010L3 is an inappropriate pharmacological tool for studying the function of native vascular K<jats:sub>V<\/jats:sub>7.1 channels in mice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.13665","type":"journal-article","created":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T14:23:13Z","timestamp":1478528593000},"page":"150-162","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Do K<sub>V<\/sub>7.1 channels contribute to control of arterial vascular tone?"],"prefix":"10.1111","volume":"174","author":[{"given":"Dmitry","family":"Tsvetkov","sequence":"first","affiliation":[{"name":"Experimental and Clinical Research Center (ECRC) A Joint Cooperation between the Charit\u00e9 Medical Faculty and the Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association (MDC) Berlin Germany"},{"name":"Department of Pharmacology and Experimental Therapy, Institute of Experimental and Clinical Pharmacology and Toxicology, Eberhard Karls University Hospitals and Clinics and Interfaculty Center of Pharmacogenomics and Drug Research University of T\u00fcbingen T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Ka\u00dfmann","sequence":"additional","affiliation":[{"name":"Experimental and Clinical Research Center (ECRC) A Joint Cooperation between the Charit\u00e9 Medical Faculty and the Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association (MDC) Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Yves","family":"Tano","sequence":"additional","affiliation":[{"name":"Experimental and Clinical Research Center (ECRC) A Joint Cooperation between the Charit\u00e9 Medical Faculty and the Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association (MDC) Berlin 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T\u00fcbingen T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian","family":"Lang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Vascular Medicine and Physiology University of T\u00fcbingen T\u00fcbingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, 223A, Lo Kwee\u2010Seong Integrated Biomedical Sciences Building, Area 39 Chinese University of Hong Kong Sha Tin Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maik","family":"Gollasch","sequence":"additional","affiliation":[{"name":"Experimental and Clinical Research Center (ECRC) A Joint Cooperation between the Charit\u00e9 Medical Faculty and the Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association (MDC) Berlin Germany"},{"name":"Medical Clinic for Nephrology and Internal Intensive Care, 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