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In this study, we have examined the effects of a barbiturate\u2010based MMP inhibitor incorporating a nitric oxide donor\/mimetic group (dinitrate\u2010barbiturate) on the intestinal injury induced by dextran sulphate sodium (DSS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p><jats:italic>In vivo<\/jats:italic>experiments were carried out using male Wistar rats given 5% DSS<jats:italic>ad libitum<\/jats:italic>in drinking water. The dinitrate\u2010barbiturate, non\u2010nitrate equivalent, nitrate side chains alone or vehicle were administered rectally, twice daily. MMP\u20109 release was measured by gelatin zymography, and analysis of gene expression was carried out using RT\u2010qPCR. TaqMan low density arrays were used to evaluate the expression of 91 inflammatory genes in the rat colon.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>The dinitrate\u2010barbiturate inhibited the induction and activity of MMP\u20109 during DSS colitis in the rat. This occurred in association with significant reductions in the colitic response to DSS as assessed by an established clinical disease activity index and a pathological colitis grade score. The compound modified expression rates of numerous inflammation\u2010related genes in the colon.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>This study demonstrated the efficacy of the dinitrate\u2010barbiturate in DSS\u2010induced colitis. Therefore, barbiturate\u2010nitrate hybrids may be developed as a promising anti\u2010inflammatory approach to the treatment of inflammatory bowel disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.13712","type":"journal-article","created":{"date-parts":[[2017,1,12]],"date-time":"2017-01-12T22:52:22Z","timestamp":1484261542000},"page":"512-524","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Inhibition of matrix metalloproteinase\u20109 by a barbiturate\u2010nitrate hybrid ameliorates dextran sulphate sodium\u2010induced colitis: effect on inflammation\u2010related genes"],"prefix":"10.1111","volume":"174","author":[{"given":"Shane","family":"O'Sullivan","sequence":"first","affiliation":[{"name":"School of Pharmacy and Pharmaceutical Sciences Trinity Biomedical Sciences Institute, Trinity College Dublin Dublin 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