{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T06:14:24Z","timestamp":1785737664157,"version":"3.56.0"},"reference-count":59,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T00:00:00Z","timestamp":1491350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["FAPESP 2013\/08216\u20102 \u2010 CRID. Christiane Becari re"],"award-info":[{"award-number":["FAPESP 2013\/08216\u20102 \u2010 CRID. Christiane Becari re"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>Angiotensin II (Ang II), whose generation largely depends on angiotensin\u2010converting enzyme (ACE) activity, mediates most of the renin\u2010angiotensin\u2010system (RAS) effects. Elastase\u20102 (ELA\u20102), a chymotrypsin\u2010serine protease elastase family member 2A, alternatively generates Ang II in rat arteries. Myocardial infarction (MI) leads to intense RAS activation, but mechanisms involved in Ang II\u2010generation in resistance arteries are unknown. We hypothesized that ELA\u20102 contributes to vascular Ang II generation and cardiac damage in mice subjected to MI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Concentration\u2010effect curves to Ang I and Ang II were performed in mesenteric resistance arteries from male wild type (WT) and ELA\u20102 knockout (ELA\u20102KO) mice subjected to left anterior descending coronary artery ligation (MI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>MI size was similar in WT and ELA\u20102KO mice. Ejection fraction and fractional shortening after MI similarly decreased in both strains. However, MI decreased stroke volume and cardiac output in WT, but not in ELA\u20102KO mice. Ang I\u2010induced contractions increased in WT mice subjected to MI (MI\u2010WT) compared with sham\u2010WT mice. No differences were observed in Ang I reactivity between arteries from ELA\u20102KO and ELA\u20102KO subjected to MI (MI\u2010ELA\u20102KO). Ang I contractions increased in arteries from MI\u2010WT versus MI\u2010ELA\u20102KO mice. Chymostatin attenuated Ang I\u2010induced vascular contractions in WT mice, but did not affect Ang I responses in ELA\u20102KO arteries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>These results provide the first evidence that ELA\u20102 contributes to increased Ang II formation in resistance arteries and modulates cardiac function after MI, implicating ELA\u20102 as a key player in ACE\u2010independent dysregulation of the RAS.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.13755","type":"journal-article","created":{"date-parts":[[2017,2,21]],"date-time":"2017-02-21T17:46:42Z","timestamp":1487699202000},"page":"1104-1115","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Elastase\u20102, an angiotensin II\u2010generating enzyme, contributes to increased angiotensin II in resistance arteries of mice with myocardial infarction"],"prefix":"10.1111","volume":"174","author":[{"given":"Christiane","family":"Becari","sequence":"first","affiliation":[{"name":"Departments of Pharmacology University of Sao Paulo  Ribeirao Preto SP Brazil"},{"name":"Physiology University of Sao Paulo  Ribeirao Preto SP Brazil"},{"name":"Department of Cardiovascular Diseases Mayo Clinic  Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcondes A B","family":"Silva","sequence":"additional","affiliation":[{"name":"Departments of Pharmacology University of Sao Paulo  Ribeirao Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina T","family":"Durand","sequence":"additional","affiliation":[{"name":"Physiology University of Sao Paulo  Ribeirao Preto SP Brazil"},{"name":"Department of Medicine University of Ribeir\u00e3o Preto  Ribeir\u00e3o Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cibele M","family":"Prado","sequence":"additional","affiliation":[{"name":"Pathology and Legal Medicine University of Sao Paulo  Ribeirao Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo B","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Biochemistry and Immunology University of Sao Paulo  Ribeirao Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauricio S","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Surgery and Anatomy, Ribeirao Preto Medical School University of Sao Paulo  Ribeirao Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helio C","family":"Salgado","sequence":"additional","affiliation":[{"name":"Physiology University of Sao Paulo  Ribeirao Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina O","family":"Salgado","sequence":"additional","affiliation":[{"name":"Departments of Pharmacology University of Sao Paulo  Ribeirao Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita C","family":"Tostes","sequence":"additional","affiliation":[{"name":"Departments of Pharmacology University of Sao Paulo  Ribeirao Preto SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,5]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13348"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13354"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-008-0336-0"},{"key":"e_1_2_12_5_1","first-page":"H1769","article-title":"Angiotensin II formation from ACE and chymase in human and animal hearts: methods and species considerations","volume":"273","author":"Balcells E","year":"1997","journal-title":"Am J Physiol"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.15.6738"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(97)00305-2"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.fjc.0000177982.68563.98"},{"key":"e_1_2_12_9_1","first-page":"E131","volume-title":"Hypertension","author":"Becari C","year":"2010"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2011007500093"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00534.2010"},{"key":"e_1_2_12_12_1","doi-asserted-by":"crossref","first-page":"A420","DOI":"10.1161\/hyp.62.suppl_1.A420","article-title":"Abstract 420: knockout mice for elastase\u20102, a novel angiotensin ii generating enzyme, displayed reduced sympathetic modulation of arterial pressure and heart rate","volume":"62","author":"Becari C","year":"2013","journal-title":"Hypertension"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(93)90098-L"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(00)00076-5"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5248.502"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autneu.2014.11.007"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12856"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104476"},{"key":"e_1_2_12_19_1","first-page":"F563","article-title":"Molecular biology of the renin\u2010angiotensin system","volume":"255","author":"Dzau VJ","year":"1988","journal-title":"Am J Physiol"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1067\/mhj.2002.120780"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(02)01689-3"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705367"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117824"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.101.8.844"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01041.2008"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00872.x"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1081\/RRS-200054355"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb15003.x"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.77.5.1001"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/ehjci\/jev014"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(80)90172-2"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.2.364"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705366"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00535a053"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.31.6.1324"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12955"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-010-0093-4"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2010.11.011"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00036.2005"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(98)00186-1"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199209033271001"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14282-1"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)00263-8"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-8807(03)00129-7"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-200205000-00002"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1139\/y02-004"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00818.2002"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1139\/y04-102"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199908193410806"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2015.02.001"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1161\/hh0901.089882"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1037"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00033.2007"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(01)00379-5"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.303587"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)09374-X"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)45712-2"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00191.2001"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12264144"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/0033-0620(82)90012-3"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbph.13755","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bph.13755","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/bph.13755","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bph.13755","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T07:55:09Z","timestamp":1693641309000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1111\/bph.13755"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,5]]},"references-count":59,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2017,5]]}},"alternative-id":["10.1111\/bph.13755"],"URL":"https:\/\/doi.org\/10.1111\/bph.13755","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,5]]},"assertion":[{"value":"2015-12-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-04-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}