{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:51:41Z","timestamp":1785851501891,"version":"3.56.0"},"reference-count":57,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2017,4,7]],"date-time":"2017-04-07T00:00:00Z","timestamp":1491523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BIM\u2010MET\/4447\/2014"],"award-info":[{"award-number":["PTDC\/BIM\u2010MET\/4447\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>Adiponectin, the most abundant peptide secreted by adipocytes, is involved in the regulation of energy metabolism and vascular physiology. Here, we have investigated the effects of exogenous administration of adiponectin on metabolism, vascular reactivity and perivascular adipose tissue (PVAT) of mesenteric arteries in Wistar rats fed a high\u2010fat diet.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>The effects of adiponectin on NO\u2010dependent and independent vasorelaxation were investigated in isolated mesenteric arteries from 12\u2010month\u2010old male Wistar rats (W12m) fed a high\u2010fat diet (HFD) for 4\u00a0months and compared with those from age\u2010matched rats given a control diet. Adiponectin ((96\u00a0\u03bcg\u00b7day<jats:sup>\u22121<\/jats:sup>) was administered by continuous infusion with a minipump, implanted subcutaneously, for 28\u00a0days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Chronic adiponectin treatment reduced body weight, total cholesterol, free fatty acids, fasting glucose and area under the curve of intraperitoneal glucose tolerance test, compared with HFD rats. It also normalized NO\u2010dependent vasorelaxation increasing endothelial NO synthase (eNOS) phosphorylation in mesenteric arteries of HFD rats. In PVAT from aged (W12m) and HFD rats there was increased expression of chemokines and pro\u2010inflammatory adipokines, the latter being important contributors to endothelial dysfunction. Infusion of adiponectin reduced these changes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>Adiponectin normalized endothelial cell function by a mechanism that involved increased eNOS phoshorylation and decreased PVAT inflammation. Detailed characterization of the adiponectin signalling pathway in the vasculature and perivascular fat is likely to provide novel approaches to the management of atherosclerosis and metabolic disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Linked Articles<\/jats:title><jats:p>This article is part of a themed section on Molecular Mechanisms Regulating Perivascular Adipose Tissue \u2013 Potential Pharmacological Targets? To view the other articles in this section visit <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bph.v174.20\/issuetoc\">http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bph.v174.20\/issuetoc<\/jats:ext-link><\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.13756","type":"journal-article","created":{"date-parts":[[2017,2,25]],"date-time":"2017-02-25T13:02:44Z","timestamp":1488027764000},"page":"3514-3526","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["Adiponectin improves endothelial function in mesenteric arteries of rats fed a high\u2010fat diet: role of perivascular adipose tissue"],"prefix":"10.1111","volume":"174","author":[{"given":"Cristina M","family":"Sena","sequence":"first","affiliation":[{"name":"Institute of Physiology, Faculty of Medicine University of Coimbra  Coimbra Portugal"},{"name":"IBILI University of Coimbra  Coimbra 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