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A growing list of proteins have been identified as MAMs components, but how they are recruited and function during complex cell stress situations is still not understood, while the participation of mitochondrial matrix proteins is largely unrecognized.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>This work compares mitochondrial\/ER contact during combined ER stress\/mitochondrial dysfunction using a model of human hepatoma cells (Hep3B cell line) treated for 24\u00a0h with classic pharmacological inducers of ER stress (thapsigargin), mitochondrial dysfunction (carbonyl cyanide m\u2010chlorophenyl hydrazone or rotenone) or both (the antiretroviral drug efavirenz used at clinically relevant concentrations).<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Markers of mitochondrial dynamics (dynamin\u2010related protein 1, optic atrophy 1 and mitofusin 2) were expressed differently with these stimuli, pointing to a specificity of combined ER\/mitochondrial stress. Lon, a matrix protease involved in protein and mtDNA quality control, was up\u2010regulated at mRNA and protein levels under all conditions. However, only efavirenz decreased the mitochondrial content of Lon while increasing its extramitochondrial presence and its localization to MAMs. This latter effect resulted in an enhanced mitochondria\/ER interaction, as shown by co\u2010immunoprecipitation experiments of MAMs protein partners and confocal microscopy imaging.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Implications<\/jats:title><jats:p>A specific dual drug\u2010induced mitochondria\u2010ER effect enhances the MAMs content of Lon and its extramitochondrial expression. This is the first report of this phenomenon and suggests a novel MAMs\u2010linked function of Lon protease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.14045","type":"journal-article","created":{"date-parts":[[2017,9,23]],"date-time":"2017-09-23T07:22:43Z","timestamp":1506151363000},"page":"4409-4429","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Lon protease: a novel mitochondrial matrix protein in the interconnection between drug\u2010induced mitochondrial dysfunction and endoplasmic reticulum stress"],"prefix":"10.1111","volume":"174","author":[{"given":"Miriam","family":"Polo","sequence":"first","affiliation":[{"name":"Departamento de Farmacolog\u00eda, Facultad de Medicina Universitat de Valencia  Valencia Spain"},{"name":"FISABIO\u2010Hospital Universitario Doctor Peset  Valencia 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