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CYP1B1 expression is increased by pro\u2010inflammatory cytokines, and it has been recently implicated in regulation of blood brain barrier function. We investigated its involvement in the increased permeability of the intestinal epithelial barrier observed in inflammatory conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Epithelial monolayers formed by human T84 colon carcinoma cells cultured on transwells, were disrupted by incubation with IFN\u03b3 (10\u00a0ng\u00b7mL<jats:sup>\u22121<\/jats:sup>). Monolayer integrity was measured using transepithelial electrical resistance. CYP1A1 and CYP1B1 inhibitors or inducers were applied apically. Potential mechanisms of action were investigated using RT\u2010qPCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>IFN\u03b3 disrupts the barrier integrity of the T84 monolayers and increases CYP1B1 and HIF1\u03b1 mRNA expression. CYP1B1 induction is inhibited by the NF\u2010\u03baB inhibitor ammonium pyrrolidinedithiocarbamate (100\u00a0\u03bcM) but not by the HIF1\u03b1 inhibitor 3\u2010(5\u2032\u2010hydroxymethyl\u20102\u2032\u2010furyl)\u20101\u2010benzyl indazole (50\u00a0\u03bcM). Inhibition of CYP1B1 with the selective inhibitor 2,4,3\u2032,5\u2032\u2010tetramethoxystilbene (100\u00a0nM) partly reverses the effects of IFN\u03b3 on epithelial permeability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>These data suggest that increased expression of CYP1B1 is involved in the effects of IFN\u03b3 on epithelial permeability. Inhibition of CYP1B1 counteracts the alterations of epithelial barrier integrity induced by IFN\u03b3 and could thus have a therapeutic potential in disorders of intestinal permeability associated with inflammation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.14122","type":"journal-article","created":{"date-parts":[[2017,12,12]],"date-time":"2017-12-12T23:22:51Z","timestamp":1513120971000},"page":"877-890","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Involvement of CYP1B1 in interferon \u03b3\u2010induced alterations of epithelial barrier integrity"],"prefix":"10.1111","volume":"175","author":[{"given":"Mireille","family":"Alhouayek","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Clinical Neuroscience, Pharmacology Unit Ume\u00e5 University Ume\u00e5 Sweden"},{"name":"Bioanalysis and Pharmacology of Bioactive Lipids Research Group, Louvain Drug Research Institute Universit\u00e9 catholique de Louvain Bruxelles Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Gouveia\u2010Figueira","sequence":"additional","affiliation":[{"name":"Department of Chemistry Ume\u00e5 University Ume\u00e5 Sweden"},{"name":"Department of Forest Genetics and Plant Physiology Swedish University of Agricultural Sciences Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010Louise","family":"Hammarstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology\/Immunology Ume\u00e5 University Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6658-7874","authenticated-orcid":false,"given":"Christopher J","family":"Fowler","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Clinical Neuroscience, Pharmacology Unit Ume\u00e5 University Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"2356","DOI":"10.4049\/jimmunol.150.6.2356","article-title":"IFN\u2010\u03b3 modulation of epithelial barrier function. 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