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We investigated the changes in expression, cellular localization and vascular inflammatory effect of B<jats:sub>1<\/jats:sub>receptors in retina of streptozotocin diabetic rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>The distribution of B<jats:sub>1<\/jats:sub>receptors on retinal cell types was investigated by immunocytochemistry. Effects of B<jats:sub>1<\/jats:sub>receptor agonist, R\u2010838, and antagonist, R\u2010954, on retinal leukocyte adhesion, gene expression of kinin and VEGF systems, B<jats:sub>1<\/jats:sub>receptor immunoreactivity, microgliosis and capillary leakage were measured. Effect of B<jats:sub>1<\/jats:sub>receptor siRNA on gene expression was also assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>mRNA levels of the kinin and VEGF systems were significantly enhanced at 2\u00a0weeks in streptozotocin (STZ)\u2010retina compared to control\u2010retina and were further increased at 6\u00a0weeks. B<jats:sub>1<\/jats:sub>receptor mRNA levels remained increased at 6\u00a0months. B<jats:sub>1<\/jats:sub>receptor immunolabelling was detected in vascular layers of the retina, on glial and ganglion cells. Intravitreal R\u2010838 amplified B<jats:sub>1<\/jats:sub>and B<jats:sub>2<\/jats:sub>receptor gene expression, B<jats:sub>1<\/jats:sub>receptor levels (immunodetection), leukostasis and vascular permeability at 2\u00a0weeks in STZ\u2010retina. Topical application (eye drops) of R\u2010954 reversed these increases in B<jats:sub>1<\/jats:sub>receptors, leukostasis and vascular permeability. Intravitreal B<jats:sub>1<\/jats:sub>receptor siRNA inhibited gene expression of kinin and VEGF systems in STZ\u2010retina. Microgliosis was unaffected by R\u2010838 or R\u2010954 in STZ\u2010retina.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Implications<\/jats:title><jats:p>Our results support the detrimental role of B<jats:sub>1<\/jats:sub>receptors on endothelial and glial cells in acute and advanced phases of DR. Topical application of the B<jats:sub>1<\/jats:sub>receptor antagonist R\u2010954 seems a feasible therapeutic approach for the treatment of DR.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.14138","type":"journal-article","created":{"date-parts":[[2017,12,29]],"date-time":"2017-12-29T08:38:58Z","timestamp":1514536738000},"page":"968-983","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Expression, distribution and function of kinin B<sub>1<\/sub>receptor in the rat diabetic retina"],"prefix":"10.1111","volume":"175","author":[{"given":"Soumaya","family":"Hachana","sequence":"first","affiliation":[{"name":"\u00c9cole d'optom\u00e9trie Universit\u00e9 de Montr\u00e9al Montr\u00e9al QC Canada"},{"name":"D\u00e9partement de Pharmacologie et Physiologie Universit\u00e9 de Montr\u00e9al Montr\u00e9al QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Menakshi","family":"Bhat","sequence":"additional","affiliation":[{"name":"\u00c9cole d'optom\u00e9trie Universit\u00e9 de Montr\u00e9al Montr\u00e9al QC Canada"},{"name":"D\u00e9partement de Pharmacologie et Physiologie Universit\u00e9 de Montr\u00e9al Montr\u00e9al QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"S\u00e9n\u00e9cal","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Pharmacologie et Physiologie Universit\u00e9 de Montr\u00e9al Montr\u00e9al QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7904-2198","authenticated-orcid":false,"given":"Fr\u00e9d\u00e9ric","family":"Hupp\u00e9\u2010Gourgues","sequence":"additional","affiliation":[{"name":"\u00c9cole d'optom\u00e9trie Universit\u00e9 de Montr\u00e9al Montr\u00e9al QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00e9jean","family":"Couture","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Pharmacologie et Physiologie Universit\u00e9 de Montr\u00e9al Montr\u00e9al QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7075-5263","authenticated-orcid":false,"given":"Elvire","family":"Vaucher","sequence":"additional","affiliation":[{"name":"\u00c9cole d'optom\u00e9trie Universit\u00e9 de Montr\u00e9al Montr\u00e9al QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,13]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705210"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjp.2008.48"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13878"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13876"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.47.12.1953"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/S0022-3565(24)36363-3","article-title":"Cardiovascular effects of Sar\u2010[D\u2010Phe8] des\u2010Arg9\u2010bradykinin, a metabolically protected agonist of B1 receptor for kinins, in the anesthetized rabbit pretreated with a sublethal dose of bacterial lipopolysaccharide","volume":"280","author":"Audet R","year":"1997","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_11_8_1","first-page":"3561","article-title":"Altered expression of retinal occludin and glial fibrillary acidic protein in experimental diabetes. 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