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However, few clinically effective and safe agents are known to promote adipocyte thermogenesis. In this study, 20 traditional Chinese herbal medicines were screened to examine whether they induced adipocyte thermogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>The effects of Chinese herbal medicines or components isolated from extracts of <jats:italic>A. membranaceus,<\/jats:italic> on adipocyte thermogenesis were analysed by assessing expression of uncoupling protein 1 (UCP1) by qPCR. Eight\u2010week\u2010old C57BL6\/J male mice were fed a high\u2010fat diet for 8\u00a0weeks and then randomized to two groups treated with vehicle or formononetin for another 8\u00a0weeks. Glucose tolerance tests and staining of adipose tissue with haematoxylin and eosin were carried out. Whole\u2010body oxygen consumption was measured with an open\u2010circuit indirect calorimetry system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Extracts of <jats:italic>A. membranaceus<\/jats:italic> increased expression of <jats:italic>Ucp1<\/jats:italic> in primary cultures of mouse adipocytes. Formononetin was the only known component of <jats:italic>A. membranaceus<\/jats:italic> extracts to increase adipocyte <jats:italic>Ucp1<\/jats:italic> expression. Diet\u2010induced obese mice treated with formononetin gained less weight and showed higher energy expenditure than untreated mice. In addition, formononetin binds directly with PPAR\u03b3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implication<\/jats:title><jats:p>Taken together, our study demonstrates that the Chinese herbal medicine from <jats:italic>A. membranaceus<\/jats:italic> and its constituent formononetin have the potential to reduce obesity and associated metabolic disorders. Our results suggest that formononetin regulates adipocyte thermogenesis as a non\u2010classical PPAR\u03b3 agonist.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.14139","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T04:27:44Z","timestamp":1515558464000},"page":"1439-1450","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["The natural compound, formononetin, extracted from <scp><i>Astragalus membranaceus<\/i><\/scp> increases adipocyte thermogenesis by modulating PPAR\u03b3 activity"],"prefix":"10.1111","volume":"175","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2111-8174","authenticated-orcid":false,"given":"Tao","family":"Nie","sequence":"first","affiliation":[{"name":"Central Laboratory of the First Affiliated Hospital of Jinan University  Guangzhou China"},{"name":"Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Joint School of Life Sciences Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  Guangzhou China"},{"name":"Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiting","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Joint School of Life Sciences Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  Guangzhou China"},{"name":"Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liufeng","family":"Mao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Joint School of Life Sciences Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  Guangzhou China"},{"name":"Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiting","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Joint School of Life Sciences Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences  Guangzhou China"},{"name":"Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoliang","family":"Lin","sequence":"additional","affiliation":[{"name":"Research and Development Centre Infinitus (China) Company Ltd.  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