{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T12:35:14Z","timestamp":1780403714916,"version":"3.54.1"},"reference-count":60,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T00:00:00Z","timestamp":1519171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Shanghai Academic Research Leader Programme","award":["16XD1403800"],"award-info":[{"award-number":["16XD1403800"]}]},{"name":"Shanghai Sailing Programme","award":["17YF1419500"],"award-info":[{"award-number":["17YF1419500"]}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>Inflammatory bowel disease (IBD) is a chronic and relapsing inflammatory disorder of the gastrointestinal tract, and an impaired immune response plays a critical role in IBD. The current drugs and therapies for IBD treatment are of limited use, therefore, there is a need to find novel drugs or therapies for this disease. We investigated the effect of cambogin in a mouse model of dextran sulphate sodium (DSS)\u2010induced colitis and whether cambogin attenuates inflammation <jats:italic>via<\/jats:italic> a Treg\u2010cell\u2010mediated effect on the immune response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Chronic colitis was established in mice using 2% DSS, and cambogin (10\u00a0mg\u00b7kg<jats:sup>\u22121<\/jats:sup>, p.o.) was administered for 10\u00a0days. Body weight, colon length and colon histology were assessed. Cytokine production was measured using <jats:sc>elisa<\/jats:sc> and quantitative real\u2010time PCR. To evaluate the mechanism of cambogin, human CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>hi<\/jats:sup>CD127<jats:sup>lo<\/jats:sup> Treg cells were isolated from peripheral blood mononuclear cells. Major signalling profiles involved in Treg cell stability were measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Cambogin attenuated diarrhoea, colon shortening and colon histological injury and IL\u20106, IFN\u2010\u03b3 and TNF\u2010\u03b1 production in DSS\u2010treated mice. Cambogin also up\u2010regulated Treg cell numbers in both the spleen and mesenteric lymph nodes. Furthermore, cambogin (10\u00a0\u03bcM) prevented Foxp3 loss in human primary Treg cells <jats:italic>in vitro<\/jats:italic>, and promoted USP7\u2010mediated Foxp3 deubiquitination and increased Foxp3 protein expression in LPS\u2010treated cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>The effect of cambogin on DSS\u2010induced colitis is expedited by a Treg\u2010cell\u2010mediated modification of the immune response, suggesting that cambogin could be applied as a novel agent for treating colitis and other Treg cell\u2010related diseases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.14150","type":"journal-article","created":{"date-parts":[[2018,1,20]],"date-time":"2018-01-20T11:52:39Z","timestamp":1516449159000},"page":"1085-1099","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Cambogin suppresses dextran sulphate sodium\u2010induced colitis by enhancing Treg cell stability and function"],"prefix":"10.1111","volume":"175","author":[{"given":"Yue","family":"Lu","sequence":"first","affiliation":[{"name":"School of Pharmacy Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na\u2010Mi","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Pharmacy Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Wen","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Pharmacy Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu\u2010Xiang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Virology and Immunology, Unit of Molecular Immunology, Institute Pasteur of Shanghai, Shanghai Institutes for Biological Sciences Chinese Academy of Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Liang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Virology and Immunology, Unit of Molecular Immunology, Institute Pasteur of Shanghai, Shanghai Institutes for Biological Sciences Chinese Academy of Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Li","sequence":"additional","affiliation":[{"name":"Unit of Molecular Immunology, Shanghai Institute of Immunology, Department of Immunology and Microbiology Shanghai JiaoTong University School of Medicine, Shanghai JiaoTong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1772-4844","authenticated-orcid":false,"given":"Hong\u2010Xi","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13882"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13880"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13876"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13877"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706826"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v23.i15.2673"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0501675102"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1132"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2000.01924.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.08.006"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa1578"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12856"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.11.7135"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2013.04.003"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.7.2099-2110.2002"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.26279"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M601770200"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa051378"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.565291"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1121"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3603-8_19"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a018341"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2017.21"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2007.01.016"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-030409-101225"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-5134"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1412881112"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00872.x"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-09-115014"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135858"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705092"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13559"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.586651"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.20050"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2013.03.008"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20061341"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2015.11.004"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.03.043"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12955"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304724200"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.8.3939"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.11.007"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2004.09.009"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.22837"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1178"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.3.1451"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.10585"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-14-1048"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200107)31:7<2170::AID-IMMU2170>3.0.CO;2-D"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M605771200"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302174"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.05.018"},{"key":"e_1_2_10_54_1","first-page":"1193","article-title":"Regulatory T cells induced by rAAV carrying the forkhead box P3 gene prevent autoimmune thyroiditis in mice","volume":"18","author":"Wang S","year":"2006","journal-title":"Int J Mol Med"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.41"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3667"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2017.03.009"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v22.i7.2195"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1401451"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2012.06.025"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1021\/np5003498"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbph.14150","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bph.14150","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T14:52:40Z","timestamp":1695653560000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1111\/bph.14150"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,21]]},"references-count":60,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1111\/bph.14150"],"URL":"https:\/\/doi.org\/10.1111\/bph.14150","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,21]]},"assertion":[{"value":"2017-07-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-12-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}