{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:07:26Z","timestamp":1790082446586,"version":"4.0.1"},"reference-count":65,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,4,14]],"date-time":"2018-04-14T00:00:00Z","timestamp":1523664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>Transient receptor potential (TRP) channels are a superfamily of non\u2010selective cation permeable channels involved in peripheral sensory signalling. Animal studies have shown that several TRPs are important players in pain modulation. Among them, the TRP melastatin 8 (TRPM8) has elicited more interest for its controversial role in nociception. This channel, expressed by a subpopulation of sensory neurons in dorsal root ganglia (DRG) and trigeminal ganglia (TG), is activated by cold temperatures and cooling agents. In experimental neuropathic pain models, an up\u2010regulation of this receptor in DRG and TG has been observed, suggesting a key role for TRPM8 in the development and maintenance of pain. Consistent with this hypothesis, TRPM8 knockout mice are less responsive to pain stimuli.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>In this study, the therapeutic potential and efficacy of two novel TRPM8 antagonists, DFL23693 and DFL23448, were tested.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Two potent and selective TRPM8 antagonists with distinct pharmacokinetic profiles, DFL23693 and DFL23448, have been fully characterized <jats:italic>in vitro<\/jats:italic>. <jats:italic>In vivo<\/jats:italic> studies in well\u2010established models, namely, the wet\u2010dog shaking test and changes in body temperature, confirmed their ability to block the TRPM8 channel. Finally, TRPM8 blockage resulted in a significant antinociceptive effect in formalin\u2010induced orofacial pain and in chronic constriction injury\u2010induced neuropathic pain, confirming an important role for this channel in pain perception.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Implications<\/jats:title><jats:p>Our findings, in agreement with previous literature, encourage further studies for a better comprehension of the therapeutic potential of TRPM8 blockers as novel agents for pain management.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.14177","type":"journal-article","created":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T16:09:07Z","timestamp":1519747747000},"page":"1691-1706","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Antinociceptive effect of two novel transient receptor potential melastatin 8 antagonists in acute and chronic pain models in rat"],"prefix":"10.1111","volume":"175","author":[{"given":"Carmen","family":"De Caro","sequence":"first","affiliation":[{"name":"Department of Pharmacy University of Naples Federico II  Naples Italy"},{"name":"Department of Science of Health, School of Medicine and Surgery University of Catanzaro  Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5950-1617","authenticated-orcid":false,"given":"Roberto","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Avagliano","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Cristiano","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Calignano","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Aramini","sequence":"additional","affiliation":[{"name":"Domp\u00e9 Farmaceutici S.p.A.  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