{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:01:47Z","timestamp":1791345707733,"version":"4.3.1"},"reference-count":38,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2018,7,3]],"date-time":"2018-07-03T00:00:00Z","timestamp":1530576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["15GRNT25910012"],"award-info":[{"award-number":["15GRNT25910012"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000849","name":"National Centre for the Replacement, Refinement and Reduction of Animals in Research","doi-asserted-by":"publisher","award":["NC\/M000079\/1"],"award-info":[{"award-number":["NC\/M000079\/1"]}],"id":[{"id":"10.13039\/501100000849","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL125572\u201001A1"],"award-info":[{"award-number":["HL125572\u201001A1"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:sec><jats:title>Background and Purpose<\/jats:title><jats:p>Platelet activation provides a critical link between inflammation and thrombosis. Sulforaphane (SFN), a naturally occurring isothiocyanate, has been shown to display both anti\u2010inflammatory and anti\u2010thrombotic actions in the systemic microvasculature. As inflammation promotes thrombosis and<jats:italic>vice versa<\/jats:italic>, in this study we investigated whether SFN is able to reduce inflammatory potentiation of thrombotic events, suppress platelet activation and thrombus formation in the cerebral microvasculature.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Approach<\/jats:title><jats:p>Thrombosis was induced in the murine brain using the light\/dye\u2010injury model, in conjunction with LPS treatment, with and without SFN treatment.<jats:italic>In vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>platelet assays (aggregation, flow and other functional tests) were also employed, using both human and murine platelets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>SFN was found to reduce LPS\u2010mediated enhancement of thrombus formation in the cerebral microcirculation. In tail\u2010bleed experiments, LPS treatment prolonged bleeding time, and SFN treatment was found to protect against this LPS\u2010induced derangement of platelet function. SFN inhibited collagen\u2010mediated platelet aggregation<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>and the associated adhesion and impaired calcium signalling. Furthermore, glycoprotein VI was shown to be involved in the protective effects observed with SFN treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Implications<\/jats:title><jats:p>The data presented here provide evidence for the use of SFN in preventing stroke in selected high\u2010risk patient cohorts.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bph.14368","type":"journal-article","created":{"date-parts":[[2018,5,24]],"date-time":"2018-05-24T07:29:18Z","timestamp":1527146958000},"page":"3333-3346","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["The isothiocyanate sulforaphane modulates platelet function and protects against cerebral thrombotic dysfunction"],"prefix":"10.1111","volume":"175","author":[{"given":"Scarlett","family":"Gillespie","sequence":"first","affiliation":[{"name":"Division of Brain Sciences Imperial College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul M","family":"Holloway","sequence":"additional","affiliation":[{"name":"Division of Brain Sciences Imperial College London London UK"},{"name":"Department of Molecular and Cellular Physiology Louisiana State University Health Sciences Center \u2013 Shreveport Shreveport LA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Becker","sequence":"additional","affiliation":[{"name":"Department of General, Visceral and Transplant Surgery University Hospital Muenster Muenster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Rauzi","sequence":"additional","affiliation":[{"name":"Platelet Biology Group, National Heart and Lung Institute Imperial College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shantel A","family":"Vital","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology Louisiana State University Health Sciences Center \u2013 Shreveport Shreveport LA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirk A","family":"Taylor","sequence":"additional","affiliation":[{"name":"Platelet Biology Group, National Heart and Lung Institute Imperial College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen Y","family":"Stokes","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology Louisiana State University Health Sciences Center \u2013 Shreveport Shreveport LA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5305-7575","authenticated-orcid":false,"given":"Michael","family":"Emerson","sequence":"additional","affiliation":[{"name":"Platelet Biology Group, National Heart and Lung Institute Imperial College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7008-5423","authenticated-orcid":false,"given":"Felicity N E","family":"Gavins","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology Louisiana State University Health Sciences Center \u2013 Shreveport Shreveport LA USA"},{"name":"Department of Neurology Louisiana State University Health Sciences Center \u2013 Shreveport Shreveport LA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13876"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13877"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1586\/erc.10.92"},{"key":"e_1_2_10_5_1","first-page":"9","article-title":"Sulforaphane protects against cardiovascular disease via Nrf2 activation","volume":"2","author":"Bai Y","year":"2015","journal-title":"Oxid Med Cell Longev"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.13.9758"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.114.304244"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH12-09-0636"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14153"},{"key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0022-3565(25)33551-2","article-title":"Mouse antithrombotic assay: a simple method for the evaluation of antithrombotic agents in vivo. 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