{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:57:40Z","timestamp":1790711860520,"version":"4.1.0"},"reference-count":67,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T00:00:00Z","timestamp":1556496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570340"],"award-info":[{"award-number":["81570340"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670344"],"award-info":[{"award-number":["81670344"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:sec>\n                    <jats:title>Background and Purpose<\/jats:title>\n                    <jats:p>\n                      Hydrogen sulfide (H\n                      <jats:sub>2<\/jats:sub>\n                      S) plays important roles as a gasotransmitter in pathologies. Increased expression of the E3 ubiquitin ligase, muscle RING finger\u20101 (MuRF1), may be involved in diabetic cardiomyopathy. Here we have investigated whether and how exogenous H\n                      <jats:sub>2<\/jats:sub>\n                      S alleviates cardiac muscle degradation through modifications of MuRF1 S\u2010sulfhydration in db\/db mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Approach<\/jats:title>\n                    <jats:p>\n                      Neonatal rat cardiomyocytes were treated with high glucose (40\u00a0mM), oleate (100\u00a0\u03bcM), palmitate (400\u00a0\u03bcM), and NaHS (100\u00a0\u03bcM) for 72\u00a0hr. MuRF1 was silenced with siRNA technology and mutation at Cys\n                      <jats:sup>44<\/jats:sup>\n                      . Endoplasmic reticulum stress markers, MuRF1 expression, and ubiquitination level were measured. db\/db mice were injected with NaHS (39\u00a0\u03bcmol\u00b7kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) for 20\u00a0weeks. Echocardiography, cardiac ultrastructure, cystathionine\u2010\u03b3\u2010lyase, cardiac structure proteins expression, and S\u2010sulfhydration production were measured.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key Results<\/jats:title>\n                    <jats:p>\n                      H\n                      <jats:sub>2<\/jats:sub>\n                      S levels and cystathionine\u2010\u03b3\u2010lyase protein expression in myocardium were decreased in db\/db mice. Exogenous H\n                      <jats:sub>2<\/jats:sub>\n                      S reversed endoplasmic reticulum stress, including impairment of the function of cardiomyocytes and structural damage in db\/db mice. Exogenous H\n                      <jats:sub>2<\/jats:sub>\n                      S could suppress the levels of myosin heavy chain 6 and myosin light chain 2 ubiquitination in cardiac tissues of db\/db mice, and MuRF1 was modified by S\u2010sulfhydration, following treatment with exogenous H\n                      <jats:sub>2<\/jats:sub>\n                      S, to reduce the interaction between MuRF1 and myosin heavy chain 6 and myosin light chain 2.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Implications<\/jats:title>\n                    <jats:p>\n                      Our findings suggest that H\n                      <jats:sub>2<\/jats:sub>\n                      S regulates MuRF1 S\u2010sulfhydration at Cys\n                      <jats:sup>44<\/jats:sup>\n                      to prevent myocardial degradation in the cardiac tissues of db\/db mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Linked Articles<\/jats:title>\n                    <jats:p>\n                      This article is part of a themed section on Hydrogen Sulfide in Biology &amp; Medicine. To view the other articles in this section visit\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bph.v177.4\/issuetoc\">http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bph.v177.4\/issuetoc<\/jats:ext-link>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bph.14601","type":"journal-article","created":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T02:49:31Z","timestamp":1549594171000},"page":"836-856","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Hydrogen sulfide regulates muscle RING finger\u20101 protein S\u2010sulfhydration at Cys\n                    <sup>44<\/sup>\n                    to prevent cardiac structural damage in diabetic cardiomyopathy"],"prefix":"10.1111","volume":"177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4165-5260","authenticated-orcid":false,"given":"Xiaojiao","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dechao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Cardiology First Affiliated Hospital of Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangping","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haining","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingzhu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyun","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanghao","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Harbin Medical University  Harbin China"},{"name":"Key Laboratory of Cardiovascular Medicine Research Harbin Medical University Ministry of Education  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01311-y"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13877"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.252742"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3741"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1065874"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","unstructured":"Bogomolovas J. 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